重组人ndrg2腺病毒对胶质瘤细胞U251抑制作用的研究

目的:研究抑癌候选基因NDRG2在体外对人脑胶质瘤细胞系U251增殖和凋亡的影响。方法:将人脑胶质瘤细胞系U251细胞分成空白对照组,重组人ndrg2腺病毒组和今又生(重组人P53腺病毒)组,重组人ndrg2腺病毒组和今又生组下设1.68×1011、8.4×1010、4.2×1010、2.1×1010、1.05×1010、5.25×109、5.25×108、5.25×107 v.p/ml组,各组分别作用96h后观察细胞形态学改变,甲基噻唑基四唑法(MTT法)绘制细胞生长抑制曲线。结果:重组人ndrg2腺病毒和今又生转染人胶质瘤细胞系U251后,细胞生长受到明显抑制,呈现明显的剂量-效应依赖曲线;重组人ndrg2腺病毒组对U251细胞的抑制率优于今又生组。结论:过表达NDRG2可明显抑制人胶质瘤细胞U251的增殖并导致细胞凋亡。

Suppressing-effects of recombinant human ad-ndrg2 on glioma cell U251

Objectiv: To investigate the function of candidate tumor suppressor gene ndrg2 on proliferation and apoptosis of human neuroblastoma cell line U251 cells.Methods: U251 cells be treated with 8 concentrations of Recombinant Human Ad-ndrg2 and Recombinant Human Ad-p53(1.68×1011、8.4×1010、4.2×1010 2.1×1010、1.05×1010、5.25×109、5.25×108、5.25×107 v.p/ml) for 96 h respectively.The morphological changes of the cells were observed under light microscopes.Cell viability was accessed by using colorimetric MTT assay.Results: As compared with normal U251 cells,the growth was markedly slowed down in ndrg2 over expressing cells and in P53 over expressing cells,Recombinant Human Ad-ndrg2 inhibited the viability of U251 cells in a dose-and effect-dependent manner.There were significant difference between the Recombinant Human Ad-ndrg2 group and the Recombinant Human Ad-p53 group respectively.Conclusion: ndrg2 gene can significantly inhibit cell proliferation and markedly induce the apoptosis of U251 cells in vitro.