地塞米松对豚鼠哮喘模型肥大细胞和类胰蛋白酶的影响

目的:探讨地塞米松对豚鼠哮喘模型肺组织中肥大细胞及血浆类胰蛋白酶含量的影响。方法:将30只雄性豚鼠分为哮喘模型、地塞米松治疗和健康对照组,用卵白蛋白(OVA)腹腔注射致敏和雾化吸入激发建立哮喘豚鼠模型。镜下观察肺组织肥大细胞数量及脱颗粒现象。运用UniCAP100全自动体外变应原检测仪测定豚鼠血浆类胰蛋白酶含量。结果:哮喘模型组豚鼠肺高倍视野肥大细胞数、脱颗粒百分率及血浆类胰蛋白酶含量分别为14.87±4.67个,42.85±5.99%,119.78±12.50均明显高于对照组(2.38±0.52个,24.91±9.79%,88.78±4.74,P<0.01);地塞米松治疗组豚鼠肺高倍视野肥大细胞数、脱颗粒百分率及血浆类胰蛋白酶含量分别为6.63±1.41个,25.33±6.14%,92.10±7.11,显著低于哮喘模型组(P<0.01)。结论:肥大细胞类胰蛋白酶参与了哮喘的致病过程;而糖皮质激素在哮喘中的抗炎作用可能是通过抑制肥大细胞类胰蛋白酶的释放而实现的。

Effects of Dexamethasone on mast cells and tryptase of asthmatic models in guinea pigs

Objective: To explore the effects of Dexamethasone on mast cells in lung tissue and tryptase in plasma of asthmatic model in guinea pigs . Methods: This experiment included 3 groups: normal control group, asthmatic model group, Dexamethasone treatment group, 10 guinea pigs involved in each group. Asthmatic models in guinea pigs were constructed by immunization of intraperitoneal injection with ovalbumin(OVA) and challenge of inspiration with nebulized OVA. Mast cells were observed under a microscope and tryptase levels were measured by antibody against tryptase with Uni CAP 100 system. Results: The numbers of mast cells and the percentage of degranulation of mast cells in lung tissue and the plasma levels of tryptase of asthmatic model group in guinea pigs 14.87±4.67, 42.85±5.99%and 119.78±12.50] were significantly higher than those of normal control group2.38±0.52, 24.91±9.79%and 88.78±4.74], P<0.01. While the above indices in the dexamethasone group6.63±1.41, 25.33±6.14%and 92.10±7.11]were significantly lower than those of the asthmatic model group(P<0.01). Conclusion: Mast cell tryptase is concerned with asthmatic process ; dexamethasone could resist inflammation, which is achieved by restraining release of mast cell tryptase.