黄芪多糖对兔动脉粥样硬化血管内皮细胞功能的影响

目的:探讨黄芪多糖对新西兰兔动脉粥样硬化内皮细胞功能的影响。方法:将40只雄性新西兰兔随机分为4组,每组10只,对照组喂普通饲料;模型组:普通饲料加质量分数为1.5%的胆固醇喂养;黄芪多糖(APS)组:普通饲料加质量分数为1.5%的胆固醇喂养,同时腹腔注射APS(12.0mg/kg);卡托普利组:普通饲料加质量分数为1.5%的胆固醇喂养,同时灌喂卡托普利(15.0mg/kg);10周后,测定各组空腹血清或血浆三脂酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、一氧化氮(NO)、内皮素-1(ET-1)、丙二醛(MDA)、C反应蛋白(CRP)含量以及超氧化物歧化酶(SOD)活性,并计算主动脉内膜粥样斑块面积。结果:模型组与对照组比较,TG、TC、MDA、ET-1、NO、CRP明显升高(P<0.01),HDL-C/TC比值、SOD活性明显下降(P<0.01),主动脉内膜粥样斑块面积较大(P<0.01);卡托普利组与模型组比较,各项指标无明显差异;而APS组与模型组比较,TG、CRP、NO、MDA、ET-1明显下降(P<0.01),TC无明显变化,HDL-C/TC比值、SOD活性明显升高(P<0.01),主动脉内膜粥样斑块面积明显减少(P<0.01)。结论:黄芪多糖对动脉粥样硬化有明显的预防和治疗作用,可能与抗氧化、免疫调节、保护血管内皮细胞有关。

The effect of astragalus polysaccharide on the function of endothelial cells in atherosclerosis rabbits

Objective:To investigate the effect of Astragalus Polysaccharide(APS) on the function of endothelial cells in atherogenesis rabbits.Methods:Foty healthy New Zealand white rabbits were randomly divided into four groops.Control groop was fed with normal diet for 10 weeks,model groop was fed with 1.5% cholesterol diet plus normal diet;APS groop was fed with 1.5% cholesterol plus 12.0mg/kg APS plus normal diet;Captopril groop was fed with 1.5% cholesterol plus 15.0mg/kg Captopril plus normal diet.After ten weeks' experiment,the concentration of each group's Triglyceride,Total Cholesterol,High Density Lipoprotein Cholesterol,C Reactive Protein,Nitric Oxide,Maleic Dialdehyde and Endothelin-1 were measured,so was the activity of superoxide dismutase.Results:Compared with the normal control group,model groups' TG,TC,NO,MDA,CRP and ET-1 increased significantly(P<0.01),and the activity of SOD and the ratio of HDL-C/TC remarkably decreased(P<0.01).Compared with the model group,each index did not have significant change in captopril groop. But compared with the model group,the APS group's TG,NO,MDA,CRP and ET-1 significantly decreased(P<0.01),while the activity of SOD and the ratio of HDL-C/TC remarkably increased(P<0.01),and TC did not show significant change.Conclusion:APS can effectively accentuate the activity of SOD against oxidation,regulate immunity and protect endothelial function against atherogenesis,so APS is a potential drug in treating and preventing atherogenesis.