酪酪肽对急性胰腺炎细菌易位的影响

目的:探讨酪酪肽(PYY)对急性胰腺炎大鼠肠粘膜的屏障功能。方法:SD大鼠40只,雌雄不拘,采用经腹腔注射精氨酸建立急性胰腺炎大鼠模型后,随机分为对照组(n=20)及治疗组(n=20),治疗组给与PYY皮下注射,治疗后第48h处死大鼠,测定肠粘膜组织匀浆丙二醛(MDA)含量、肠粘膜蛋白含量以及肠管细菌易位率。结果:治疗组动物肠粘膜蛋白含量显著高于对照组(P<0.01);肠粘膜组织匀浆MDA含量显著低于对照组(P<0.01);肝脏及脾脏细菌培养阳性率显著低于对照组(P<0.05)。结论:PYY对急性胰腺炎大鼠肠黏膜屏障具有保护作用。

The effect of Peptide YY on bacterial translocation in rat with acute pancreatitis

Objective: To evaluate the effect of Peptide YY on intestinal barrier function in rats with acute pancreatitis.Methods: 40 Sprague-Dawley rats received injection of 6 % L-Arginine(1.2mg/g×3) into the belly cavity and were randomly divided into control group(n=20) and treatment group(n=20).Animals in treatment group were injected PYY at a dose of 0.8pmol·g-1·4h-1.The rats were sacrificed 48 hours after treatment.Protein and MDA content in ileum mucosa were determined;mesenteric lymph nodes,pancreas,liver,spleen were harvested for cultures.Results: Protein content in ileum mucosa in treatment group was significantly higher than in control group(P<0.01).The ileum mucosa homogenate levels of MDA were significantly lower than in control group(P<0.01).Positive cultures in liver and spleen in treatment group were significantly lower than in control group(P<0.05).Conclusions: Peptide YY may prevent the increase of bacterial translocation in rats with acute pancreatitis.