比较结核感染T细胞斑点试验、结核菌素试验以及血清结核抗体检测对诊断肺结核的价值

目的：比较结核感染T细胞斑点试验(T-cell spot of tuberculosis,T-SPOT.TB)、结核菌素试验(purified protein derivative,PPD)以及血清结核抗体(Tuberculosis antibody,TB-Ab)检测对肺结核的诊断价值。方法随机选取自2014年1月—2016年8月在该院确诊为肺结核患者60例,作为肺结核组；确诊非肺结核就诊者30名作为非肺结核组,两组就诊者均接受了T-SPOT.TB、PPD以及TB-Ab检测。比较两组接受不同检测方法对诊断肺结核灵敏性与特异性的差异。结果 T-SPOT.TB检测诊断肺结核的敏感性为73.30%,特异性为80.00%；PPD试验诊断肺结核的敏感性为60.00%,特异性为86.70%；血清TB-Ab检测检测诊断肺结核的敏感性为53.30%,特异性为66.70%。结论 T-SPOT.TB诊断肺结核的敏感性高于PPD试验和血清结核抗体检测；T细胞斑点试验T-SPOT.TB诊断肺结核的特异性高于血清结核抗体检测,与PPD试验差异无统计学意义。

Comparison of T-SPOT.TB,PPD, and TB-Ab Detection in the Diagnosis of Pulmonary Tuberculosis

Objective To compare of T-cell spot of tuberculosis (T-SPOT.TB), purified protein derivative test (PPD), and serum tuberculosis antibody detection (TB-Ab) in the diagnosis of pulmonary tuberculosis. Methods 90 patients from Jan-uary 2014 to August 2016 in our hospital were random selected and divided into pulmonary tuberculosis group (n=60) and non-tuberculosis group (n=30). The positive rates of T-SPOT.TB, PPD, and TB-Ab in two groups were detected and their sensitivities and specifities were compared. Results The sensitivity of T-SPOT.TB in the diagnosis of pulmonary tuberculo-sis was 73.30%, and the specificity was 80.00%; The sensitivity of PPD test in the diagnosis of pulmonary tuberculosis was 60.00%, and the specificity was 86.70%; The sensitivity of TB-Ab detection in the diagnosis of pulmonary tuberculosis was 53.30% and specificity was 66.70%. Conclusion The sensitivity of T-SPOT.TB in the diagnosis of tuberculosis is higher than that of PPD test and TB-Ab detection; the specificity of T-SPOT.TB in diagnosis of pulmonary tuberculosis is higher than that of serum TB-Ab detection, and is no significant difference with PPD test.