| 靶向La的外源性microRNA抑制HBV复制和表达的实验研究 |
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| 引用本文： | 郜玉峰,叶珺,邹桂舟,余莉,沈继龙,李家斌,李旭.靶向La的外源性microRNA抑制HBV复制和表达的实验研究[J].安徽医科大学学报,2011,46(1):11-15. |
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| 作者姓名： | 郜玉峰 叶珺 邹桂舟 余莉 沈继龙 李家斌 李旭 |
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| 作者单位： | 安徽医科大学第二附属医院肝病科,合肥,230601;安徽省人兽共患病实验室,教育部省部共建重点实验室,合肥,230032;安徽医科大学第一附属医院感染病科,合肥,230032 |
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| 基金项目： | 国家自然科学基金(编号:30700698) |
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| 摘 要： | 目的 设计并构建靶向La的microRNA表达载体,观察其对La基因的抑制作用,探讨其在HBV感染基因治疗中的应用价值.方法 以La为靶基因,设计并构建3个针对La不同位点的microRNA表达载体,通过脂质体方法转染HepG2.2.15细胞,RT-PCR和Western blot检测其对La mRNA和蛋白的抑制作用...
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| 关 键 词： | 肝炎病毒 乙型 RNA干扰 |
Inhibition of hepatitis B virus gene replication and expression by artificial microRNA targeted La gene |
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| Gao Yufeng,Ye Jun,Zou Guizhou,et al.Inhibition of hepatitis B virus gene replication and expression by artificial microRNA targeted La gene[J].Acta Universitis Medicinalis Anhui,2011,46(1):11-15. |
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| Authors: | Gao Yufeng Ye Jun Zou Guizhou |
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| Institution: | Gao Yufeng,Ye Jun,Zou Guizhou,et al(Dept of Hepatopathy,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601) |
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| Abstract: | Objective To investigate the inhibitory effects of hepatitis B virus(HBV)replication and expression by transfecting artificial microRNA targeted La gene into HepG2.2.15 cells.Methods Three amiRNA-HBV plasmids were constructed and transfected into HepG2.2.15 cells via LipofectamineTM 2000 reagent.The level of La mRNAand La protein was measured by semi-quantitative RT-PCR and Western blot respectively.HBV antigen secretion was detected in the cells with transient and stable transfection by time-resolved fluor... |
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| Keywords: | virus hepatitis B RNA interference |
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