TLR7激动剂Gardiquimod上调BxPC-3细胞中IL-15 mRNA表达

目的：研究TLR7在胰腺癌BxPC-3细胞中表达,并探讨TLR7激动剂激活TLR7后细胞因子白介素15( IL-15)的表达。方法通过 Western blot 和 Real-time PCR 分析TLR7在细胞内的表达水平；BxPC-3细胞经过不同时间点Gardiquimod(3μg/ml)的处理后,Real-time PCR 分析 TLR7激活后 IL-15 mRNA 水平表达变化；Western blot 分析Gardiquimod刺激细胞后,磷脂酰肌醇-3-激酶-蛋白质丝氨酸苏氨酸激酶( PI3K-AKT)信号通路的变化。结果 Western blot和 Real-time PCR结果显示：与外周血单核细胞( PBMC)相比, TLR7在 BxPC-3中弱表达；Real-time PCR分析显示：Gardiquimod处理细胞后能刺激细胞中IL-15的表达；TLR7激动剂能够激活 PI3K-AKT信号通路。结论 Gardiquimod激活TLR7后能够上调 IL-15的表达,并且其激活与 PI3K-AKT信号途径相关。

The agonist of TLR7 Gardiquimod up-regulates the expression of interleukin-15 in BxPC-3 cells

Objective To investigate the expression of Toll-like receptor 7 ( TLR7 ) in pancreatic cancer BxPC-3 cells, and to explore the effect of TLR7 activation on the expression of interleukin-15(IL-15). Methods BxPC-3 cells were used to analyze the expression of TLR7 by Western blot and Real-time PCR. The cells were treated with Gardiquimod (3 μg/ml) at different times, the expression of IL-15 at mRNA level by Real-time PCR. Western blot was performed to analyze the phosphorylation level changes of phosphatidyl inositol kinase serine/threonine kinase (PI3K-AKT) protein in BxPC-3 cells stimulated by TLR7 ligand Gardiquimod. Results Western blot and Real-time PCR results showed that compared with peripheral blood mononuclear cells ( PBMC ) , TLR7 presented weak expression in BxPC-3 cells. Gardiquimod could increase the expression of IL-15 in BxPC-3 cells. TLR7 agonist could activate the PI3K-AKT signaling pathway. Conclusion Gardiquimod can up-regulate the expression of IL-15 and this effect can be associated with PI3K-AKT signaling pathway.