| 哮喘大鼠模型支气管肺组织骨桥蛋白表达的变化及其调控机制的初探 |
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| 引用本文： | 齐胤良,桂淑玉,王勇生,张素梅,何苇,周青,汪渊.哮喘大鼠模型支气管肺组织骨桥蛋白表达的变化及其调控机制的初探[J].安徽医科大学学报,2010,45(1):9-12. |
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| 作者姓名： | 齐胤良 桂淑玉 王勇生 张素梅 何苇 周青 汪渊 |
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| 作者单位： | 安徽医科大学第一附属医院呼吸内科,合肥,230022;巢湖市第一人民医院呼吸内科,巢湖,238000;安徽医科大学分子生物学实验室,合肥,230032 |
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| 基金项目： | 安徽省自然科学基金资助项目 |
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| 摘 要： | 目的 研究哮喘大鼠模型支气管肺组织骨桥蛋白(OPN)表达变化及其调控机制;探讨地塞米松(DXM)对哮喘大鼠模型支气管肺OPN表达的影响及其机制.方法 SD大鼠24只,随机分为正常对照组(A组)、哮喘模型组(B组)、DXM治疗组(C组).以卵蛋白(OVA)致敏和激发建立大鼠支气管哮喘模型,肺组织切片用于HE染色以观察支气管组织学变化;用免疫组织化学和免疫印迹法分析三组大鼠支气管肺组织中OPN的表达及ERK、p38的磷酸化水平.结果 B组大鼠支气管肺组织OPN表达较A组增加,DXM对其表达有明显抑制作用(P<0.05);B组支气管肺组织ERK磷酸化水平较A组明显增加,DXM对其磷酸化有明显抑制作用(P<0.05);而支气管肺组织p38磷酸化水平在B组与A组之间无明显变化,但C组p38磷酸化水平明显下降.结论 DXM对OPN表达的增加有抑制作用,其可能的机制是通过ERK信号通路影响OPN的表达.
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| 关 键 词： | 哮喘 骨桥蛋白质 大鼠 |
The change of osteopontin expression in bronchial lung tissue of asthma rat model and its probably regulatory mechanism |
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| Qi Yinliang,Gui Shuyu,Wang Yongsheng,et al.The change of osteopontin expression in bronchial lung tissue of asthma rat model and its probably regulatory mechanism[J].Acta Universitis Medicinalis Anhui,2010,45(1):9-12. |
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| Authors: | Qi Yinliang Gui Shuyu Wang Yongsheng |
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| Institution: | Qi Yinliang1,Gui Shuyu1,Wang Yongsheng2,et al(1Dept of Respiratory Medicine,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,2Laboratory of Molecular Biology,Anhui Medical University,Hefei 230032) |
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| Abstract: | Objective To investigate the expression of osteopontin(OPN)in bronchial lung tissue of asthma rat model and the regulatory mechanism,and the influence of dexamethasone on OPN expression in bronchial lung tissue of asthma rat model.Methods Twenty-four SD rats were randomly divided into control group(group A),asthma model group(group B),DXM group(group C).Asthma rat model was established by sensitization and stimulation with ovalbumin(OVA).Bronchial lung tissue slices were stained with HE to observe the histo... |
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| Keywords: | asthma osteopontin rats |
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