结直肠癌细胞增殖核抗原及生长抑制因子4表达的关系及预后因素

目的 探讨细胞增殖核抗原(Ki-67)和生长抑制因子 4(ING4)在结直肠癌发病机制中的作用及其对患者预后的影响.方法 应用免疫组织化学法检测71例结直肠癌组织和30例癌旁正常组织中Ki-67、ING4的表达情况,分析其表达与临床病理特征及预后的关系.结果 Ki-67在癌组织中阳性表达率(85. 9％)高于癌旁组织(6.7％)(P＜0.05), ING4在癌组织中阳性表达率(56. 3％)低于癌旁组织(90％)(P＜0.05). Ki-67表达与肿瘤浸润深度、有无淋巴结转移、淋巴结转移数目、TNM分期有关(P＜0.05), ING4表达与分化程度、有无淋巴结转移、淋巴结转移数目、TNM有关(P＜ 0.05).Ki-67、ING4在结直肠癌组织中表达呈负相关性(r=-0.428,P＜0.05).Ki-67高表达组和低表达组术后5年生存率分别为63. 9％,85. 5％,差异有统计学意义(P＜0.05);ING4阳性组和阴性组术后5年生存率分别为 97. 4％ 、45. 2％,差异有统计学意义(P＜0.05).Cox多因素分析结果显示,TNM分期、ING4表达情况均为影响患者预后的独立因素.结论 Ki-67、ING4可能参与直肠癌的发生和发展,ING4是影响结直肠癌患者预后的独立危险因素.

Relationship between Ki-67 and ING4 expression and their prognostic significance in colorectal cancer

Objective To investigate the expression of Ki-67 and ING4 and their influence on survival in patients with colorectal cancer. Methods The expressions of Ki-67 and ING4 proteins were detected by immunohistochemistry in colorectal cancer tissues from 71 cases of colorectal cancer and normal colorectal tissues from 30 corresponding para-cancerous normal tissues. The correlations between the expressions of Ki-67, ING4 and clinicopathological parameters and prognostic significance were also analyzed. Results The colorectal cancer tissues's positive expression rates of Ki-67 was 85. 9％, which was significantly higher in innormal tissue group (6. 7％ ) (P ＜ 0. 05 ), The colorectal cancer tissues's positive expression rates of ING4 was 56. 3％, which was significantly lower in innormal tissue group (90％ ) (P ＜ 0. 05). Ki-67 expressions in colorectal cancer tissues were correlated with infiltration depth, lymph node metastasis, number of lymph node metastasis and TNM staging (P ＜ 0. 05 ). ING4 expressions in colorectal cancer tissues were correlated with histological grade, lymph node metastasis, number of lymph node metastasis and TNM staging (P ＜ 0. 05). Ki-67 was negatively correlated with ING4(r =-0.428, P＜0. 05). The 5-year survival rate in the Ki-67 positive group (63. 9％ ) was lower compared with the Ki-67 negative group(85. 5％ ) (P ＜0. 05 ). The 5-year survival rate in the ING4 positive group(97. 4％ ) was higher compared with the ING4 negative group (45. 2％ )(P ＜0. 05). The multivariate analysis showed that TMN stage and ING4 are independent prognostic factors in colorectal cancer. Conclusion Ki-67 and ING4 may be involved in the occurrence and development of colorectal cancer, and ING4 is independent factors affecting the prognosis in patient with colorectal cancer.