海藻酸钠壳聚糖支架复合成骨细胞特异性多肽修复兔颅骨缺损

目的 评价成骨细胞特异性识别多肽在骨缺损中的修复效果.方法 将成骨细胞特异性识别多肽与海藻酸钠/壳聚糖水凝胶(SA/CS Gel)复合植入兔颅骨双侧 15 mm圆形极限骨缺损区,实验组缺损处植入海藻酸钠/壳聚糖/成骨细胞特异性识别多肽水凝胶(SA/CS/多肽 Gel),对照组植入SA/CS Gel,空白组不植入任何载体及药物,随机分组.术后8周活体行CBCT扫描,随后处死取材并常规HE染色行组织学检查观察成骨效果.结果 影像学及组织形态学结果可见,实验组缺损区成骨量明显大于对照组及空白组,差异有统计学意义(P<0.05).结论 成骨细胞特异性识别多肽可促进兔颅骨缺损的骨修复.

The effects of sodium alginate and chitosan composite hydrogels combination with the peptide that specifically bind to on the repair of rabbit calvarial defectsosteoblasts

Objective To evaluate the effect of the peptide that specifically bind to osteoblasts on bone healing.Methods To enhance bone regeneration of the rabbit calvarial defect, sodium alginate and chitosan composite hygrogels(SA/CS Gel) were used as scaffold to load the peptide that specifically bind to osteoblasts.Bilateral parietal critical size bone defects of 15 mm diameter were performed in rabbits, all defects were randomly divided into SA/CS/Peptide Gel group, SA/CS Gel group and the empty group.The bone regeneration ability of the peptide that specifically bind to osteoblasts was investigated by CBCT.Hematoxylin and eosin staining(HE) was performed at 8 weeks after surgery.Results According to imaging and histomorphology, SA/CS/Peptide group showed the highest bone regeneration than SA/CS Gel group and the empty group (P<0.05).Conclusion The peptide that specifically bind to osteoblasts can enhance the bone regeneration of rabbit calvarial defect.