采用高速离心及ExoQuick-TC法提取小鼠骨髓间充质干细胞来源外泌体比较及鉴定 |
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引用本文: | 韩金秀,李宏远,撒亚莲,严丹,贺继刚.采用高速离心及ExoQuick-TC法提取小鼠骨髓间充质干细胞来源外泌体比较及鉴定[J].安徽医科大学学报,2017,52(4). |
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作者姓名: | 韩金秀 李宏远 撒亚莲 严丹 贺继刚 |
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作者单位: | 昆明理工大学附属云南省第一人民医院心脏大血管外科,昆明,650032;昆明理工大学附属云南省第一人民医院基础研究所,昆明,650032;昆明理工大学附属云南省第一人民医院重症医学科,昆明,650032 |
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基金项目: | 国家自然科学基金,云南省科技厅-昆明医科大学应用基础研究联合专项,云南省教育厅科学研究基金,中国博士后科学基金,成都医学院校级课题 |
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摘 要: | 比较及鉴定超高速离心法及ExoQuick-TC法提取小鼠骨髓间充质干细胞来源外泌体.采用BCA蛋白检测可见ExoQuick-TC法提取的外泌体蛋白含量高于超高速离心法提取的蛋白含量.Exosome Antibodies,Array & ELISA Kits检测可见ExoQuick-TC法提取外泌体的纯度优于超高速离心法提取的纯度.两种方法提取的外泌体电镜下形状无差异,随机选取3个电镜视野进行计数可见ExoQuick-TC法提取外泌体数量多于超高速离心法提取外泌体. ExoQuick-TC法提取的外泌体具有更高的产量及纯度.
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关 键 词: | 外泌体 小鼠骨髓间充质干细胞 超高速离心 ExoQuick-TC法 |
Comparison of ultracentrifugation and ExoQuick-TC for the identificationof exosomes extracted from mouse bone marrow mesenchymal stem cells |
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Abstract: | To compare ultracentrifugation and ExoQuick-TC in identifying the characteristics of exosomes extracted from mouse bone marrow mesenchymal stem cells.BCA protein assay revealed that the protein contents in the ExoQuick-TC-extracted exosomes were higher than those in the ultracentrifugation-extracted exosomes.Analysis using exosome antibodies, array, and ELISA kits indicated that the purity of the ExoQuick-TC-extracted exosomes was higher that of the ultracentrifugation-extracted exosomes.Electronmicroscopy demonstrated that the morphological characteristics of the ExoQuick-TC-extracted exosomes did not significantly differ from those of the ultracentrifugation-extracted exosomes.Three electron microscope visual fields were randomly selected to count the number of exosomes.Results showed that the number of the ExoQuick-TC-extracted exosomes was more than that of the ultracentrifugation-extracted exosomes.Therefore, the production and purity of ExoQuick-TC-extracted exosomes are higher than those of ultracentrifugation-extracted exosomes. |
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Keywords: | exosome mouse bone marrow mesenchymal stem cells ultra-high speed centrifuge ExoQuick-TC |
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