睡眠剥夺对健康成人学习记忆网络影响的功能磁共振研究

目的 探讨36 h完全睡眠剥夺对健康受试学习记忆网络的影响.方法 利用血氧水平依赖功能磁共振成像技术分别采集18例健康成年男性正常睡眠状态及36 h完全睡眠剥夺后静息态功能磁共振数据,利用REST软件自动化解剖标记模板,选取双侧海马为种子点做功能连接,再以SPM 8软件进行配对t检验,比较睡眠剥夺前后双侧海马与全脑功能连接的变化.最后将功能连接显著的脑区与韦氏记忆量表的记忆商差值(睡眠剥夺后减去正常睡眠状态)做相关分析.结果 与睡眠剥夺前对比,36 h睡眠剥夺后:双侧海马种子点主要与右侧后扣带回皮质呈显著正功能连接;与双侧中央后回呈显著负功能连接.与睡眠剥夺前相比,韦氏记忆量表的记忆商显著降低,功能连接与韦氏记忆量表的记忆商差值相关分析显示:双侧海马种子点与右侧后扣带回皮质间功能连接与韦氏记忆量表的记忆商差值之间呈负相关性(左侧:r=-0.463,P=0.015;右侧:r=-0.546,P=0.003).结论 与正常睡眠状态相比,睡眠剥夺对双侧海马功能连接存在显著影响,主要表现为与右侧扣带回皮质间功能连接显著上升,而与双侧中央后回间功能连接显著下降.其中前者可能是睡眠剥夺后学习记忆能力调控方面的重要神经机制.

Effect of sleep deprivation on learning and memory network in healthy adults by fMRI

Objective To observe the neural mechanism of learning and memory network disturbance induced by 36 hours sleep deprivation(SD).Methods 18 young male healthy volunteers were enrolled in this study.Resting state functional magnetic imaging(rs-fMRI) data was collected by blood oxygen level dependence functional magnetic imaging(BOLD-fMRI) during rest wakefulness(RW) and 36 hours SD.Bilateral hippocampus were selected as seed points by REST software automated anatomical labeling template.And then compared the differences of functional connectivity(FC) between bilateral hippocampus and whole brain regions by using paired-t test within statistical parametric mapping 8 (SPM8) software.Finally,correlation analysis had been done between significant FC of brain regions and WMS MQ values (SD-RW).Results Compared with RW,the bilateral hippocampus showed positive connected with the right dorsal posterior cingulate cortex,and showed negative functional connectivity with bilateral postcentral gyrus.The WMS MQ was significantly decreased after 36 hours SD.The connection between bilateral hippocampus and the right posterior cingulate cortex showed negative correlation with the deductionofWMS MQ (left:r=-0.463,P=0.015;right:r=-0.546,P=0.003).Conclusion SD has a significant effect on bilateral hippocampal FC.The FC between bilateral hippocampus and right dorsal posterior cingulate cortexis significant increased,while between bilateral postcentral gyrus is decreased after 36 hours SD.The former may be an important neurological mechanism in learning and memory ability regulation after 36 hours sleep deprivation.