bmp-2和vegf165共表达基因修饰的BMSCs对腱骨界面愈合的研究

目的 探讨骨形态发生蛋白-2(bmp-2)和血管内皮生长因子165(vegf165)共表达基因修饰的骨髓间充质干细胞(BMSCs)对兔前交叉韧带重建术后腱骨界面的组织形态学和生物力学的影响.方法 将66只大白兔,随机分成实验组、对照组及正常组.实验组术后在腱骨界面注入经慢病毒转染稳定表达bmp-2和vegf165的BMSCs和纤维蛋白胶(FG),对照组腱骨界面注入BMSCs和FG,分别在术后第3、8和12周进行组织学观察和生物力学检测.结果 组织学病理显示,实验组新生血管和成纤维细胞浸润高于对照组,并在术后第12周形成典型的4层直接止点结构,其组织愈合情况优于同期对照组.生物力学显示相同时间点实验组最大载负荷明显高于对照组,差异有统计学意义(P<0.05).结论 bmp-2和vegf165共表达基因修饰的BMSCs对腱骨界面愈合有促进作用.

Effect of modified BMSCs with gene co-expression ofbmp2 and vegf165 on tendon-bone healing

Objective To investigate the effect of modified bone mesenchymal stem cells (BMSCs) with gene co-expression of bone morphogenetic protein 2 (bmp2) and vascular endothelial growth factor 165 (vegf165) on histomorphology and biomechanics of tendon-bone healing after rabbit anterior cruciate ligament reconstruction.Methods Sixty-six white rabbits were randomly divided into three groups: experimental group, control group, normal group.The tendon-bone interface was injected with BMSCs transfered by lentiviral vector with gene co-expression of bmp2 and vegf165 in experimental group, BMSCs without transfection in control group and none in normal group.Histomorphology and biomechanics were evaluated in 3rd, 8th and 12th week after surgery.Results Histological pathology revealed that neovascularization and fibroblast cells infiltration in experimental group were higher than that in the control group.Ability of tissue healing was better than the control group owing to the formation of typical 4-layered tendon-bone interface at in 12th week postoperatively.Biomechanics test found that the maximum loads in the experimental group showed significant superior outcomes compared with the control group (P<0.05).Conclusion The modified BMSCs with gene co-expression of bmp2 and vegf165 in experimental group, BMSCs without transfection in control group and none in normal group.Histomorphology and biomechanics were evaluated in 3rd, 8th and 12th week after surgery. Results　Histological pathology revealed that neovascularization and fibroblast cells infiltration in experimental group were higher than that in the control group. Ability of tissue healing was better than the control group owing to the formation of typical 4-layered tendon-bone interface at in 12th week postoperatively. Biomechanics test found that the maximum loads in the experimental group showed significant superior outcomes compared with the control group (P <0． 05).Conclusion The modified BMSCs with gene co-expression of bmp2 and vegf165 may improve the outcome of tendon-bone healing.