| 人脂肪干细胞与聚L-谷氨酸/海藻酸钠可注射水凝胶的生物相容性研究 |
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| 引用本文： | 于文渊,崔磊,赵宇,颜世峰,朱捷.人脂肪干细胞与聚L-谷氨酸/海藻酸钠可注射水凝胶的生物相容性研究[J].安徽医科大学学报,2012,47(6):727-731. |
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| 作者姓名： | 于文渊 崔磊 赵宇 颜世峰 朱捷 |
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| 作者单位： | 安徽医科大学第一附属医院整形外科,合肥,230022;上海交通大学医学院附属第九人民医院整复外科,上海,200011;上海大学高分子化学与物理实验室,上海,200436 |
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| 基金项目： | 安徽省教育厅自然科学基金重大项目 |
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| 摘 要： | 天然高分子聚合物聚L-谷氨酸(PLGA)与氧化海藻酸钠(OAg)共价交联合成PLGA/OAg水凝胶,将人脂肪来源干细胞(hADSCs)接种于水凝胶内,倒置显微镜、扫描电镜观察细胞在支架中的存活、增殖、黏附情况,DNA定量法检测细胞的增殖。体外证实该水凝胶材料生物相容性良好,具有性能可控性和微创性等优势,对于进一步开发脂肪组织工程材料具有指导性意义。
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| 关 键 词： | 人脂肪干细胞 水凝胶 可注射支架 脂肪组织工程 |
Biocompatibility research of hADSCs cultivated in PLGA/OAg hydrogel in vitro |
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| Institution: | Yu Wenyuan1,Cui Lei2,Zhao Yu1,et al(1Dept of Plastic Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022; 2Shanghai Ninth People′s Hospital Affiliated Shanghai JiaoTong University School of Medicine,Shanghai 200011) |
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| Abstract: | PLGA/OAg hydrogel was fabricated by poly L-glutamic acid(PLGA) and oxidation sodium alginate(OAg) covalent crosslinking.HADSCs were encapsulated into the PLGA/OAg hydrogel scaffolds and cultivated in three-dimensional environment in vitro to observe biocompatibility of the scaffolds include cell viability,adhesion,proliferation by inverted microscope and scanning electron microscopy.The cell proliferation was tested by DNA quantitative method.The synthetical hydrogel performances an excellent biocompatibility,controllability and minimally invasive advantages.It may be an instructive fat tissue engineering material in the future. |
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| Keywords: | adipose derived stem cell hydrogel injectable scaffold adipose tissue engineering |
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