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瓜蒌薤白颗粒中多糖的含量测定
引用本文:鄢海燕,邹纯才.瓜蒌薤白颗粒中多糖的含量测定[J].皖南医学院学报,2012(5):363-365.
作者姓名:鄢海燕  邹纯才
作者单位:皖南医学院药剂学与生药学教研室;皖南医学院药物分析与药物化学教研室
基金项目:安徽高校省级自然科学研究项目(KJ2012B201)
摘    要:目的:从瓜蒌薤白颗粒中提取多糖并测定其含量。方法:以葡萄糖为对照品,采用硫酸-苯酚法测定瓜蒌薤白颗粒中多糖含量。结果:标准曲线回归方程为A=7.16C-0.0124,r=0.9998,加样回收率为98.04%,RSD=2.80%。结论:该法操作简单,重复性较好,可作为瓜蒌薤白颗粒中多糖含量测定的方法。

关 键 词:瓜蒌薤白颗粒  多糖  含量测定

Content determination of polysaccharide in Gualou-Xiebai granules
YAN Hai-yan,ZOU Chun-cai.Content determination of polysaccharide in Gualou-Xiebai granules[J].Acta Academiae Medicinae Wannan,2012(5):363-365.
Authors:YAN Hai-yan  ZOU Chun-cai
Institution:Department of Pharmacy and Pharmacognosy,Wannan Medical College,Wuhu 241002,China
Abstract:Objective:To extract the polysaccharide from the compound granules of Gualou and Xiebai(Fructus Trichosanthis and Bulbus Allii Macrostemi,Chinese medicinal herbs) and determine its content.Methods:Phenol-sulfuric acid method was used to determine the content of polysaccharide with glucose as reference substance.Results: The standard curve regression equation was:A=7.16C-0.0124,r=0.9998,and the recovery of the added sample was 98.04%,with relative standard deviation(RSD) of 2.80%.Conclusion: This technique can be used for determining the content of polysaccharide in Gualou-Xiebai granules for its simple performance and better reproducibility.
Keywords:Gualou-Xiebai granules  polysaccharide  content determination
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