氯化汞对K562和HEL细胞株作用的实验研究

为探讨中药水银（Ｈｇ）和经粉（ＨｇＣｌ）的抗肿瘤作用，以三氧化二砷（Ａｓ２Ｏ３）及阿糖胞苷（Ａｒａ－ｃ）为对照，应用细胞形态学、流式细胞术、ＭＴＴ法和药物反应曲线等方法观察３种药物对Ｋ５６２和ＨＥＬ细胞株的作用。结果表明，ＨｇＣｌ２和Ａｓ２Ｏ３均能诱导Ｋ５６２细胞凋亡，而Ａｒａ－ｃ无此作用。ＨｇＣｌ２可使Ｋ５６２的Ｇ０／Ｇ１增高，揭示激活Ｐ５３基因可能是氯化汞诱导凋亡机制之一；ＨｇＣｌ２和Ａｓ２Ｏ

Effects of mercuric chloride (HgCl_2) on K_(562) and HEL cell lines

To explore the effects of HgCl2 on K562 and HEL cell lines, and compared it with HgCl2 AS2O3 and Ara-c by using morphology, flow cytometry, MTT and drug-reactive curve. The results indicated that both HgCl2 and As2O3 could induce K562 cells apoptosis ,Ala-c did not have these effects; HgCl2 could increase percentage of K56, cells in G0/G1 phase. It indicated that HgCl2 induces K562 cells apoptosis probably by triggering P53 gene; As2O3 and HgCl2 inhibited the proliferation of K562 cells significantly; the proliferation of HEL cells was inhibited by Ara-c notably although it is not sensitive to As2O3 and HgCl2 .However, they all can not induce the apoptosis of HEL cell. HgCl2 is a new drug that can induce leukemia cells apoptosis.