环组己酰胺诱导肿瘤逆分化过程中癌相关抗原改变的研究

我们应用2.5～5.0mmol/L的CAHB诱导Blu-87膀胱癌细胞和LAH-GC-1,SGC-7901胃癌细胞分化120小时后,经ABC染色,发现Blu-87细胞表面BB369相关抗原和LAH-GC-1、SGC-7901细胞表面1D1.2相关抗原的丢失率分别是94.2％、72.9％和74.0％,与对照组比较差异有非常显著性意义(P<0.01)。原代培养的膀胱癌及胃癌细胞,在诱导120小时后,癌细胞表面BB369和1D1.2相关抗原的丢失率分别是93.4％和100％。

yclic Assembled Hexamethlene Bisacetamide Induced Carcinoma to Lose the BB369 and 1D1,2 Epitopes

Human bladder cancer cell line BIU -87 cells and the gastric cancer cell lines LAH-GC-1 ,SGC-7901 cells together with the primary cultures of bladder transitional cell cancer as well as gastric carcinoma were exposed to 2. 5 to 5. 0 mM of cyclic assembled hexamethylene bisacetamide (CAHB) for 5 days, then the cancer cell associated antigens were identified by monoclonal antibodies BB369 or 1D1,2 respectively using immunoperioxidase staining. The results shown that the lost of BB369 associated antigen shed by BIU-87 cell and the 1D1.2 antigen shed by LAH-GC-1 and SGC-7901 cells that was disappered were 94. 2% ,72. 9%, 74. 0% respectively, which are significant difference from controlled groups (P<0. 001). For the primary cultures of cancer cells,the BB369 and 1D1,2 antigens loss on bladder cancer or gastric cancer cells were 93. 4% and 100% respectively. These results indicate that the presence of shed antigen in urine or specimen may have diagnostic and evaluation of cancer therapy potential.