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膜分离与沉淀剂结合提取腌肠衣盐水中肝素纳的工艺研究
引用本文:李耀曾,曹芸,夏华,姚世霞,赵建邦,杜兴,张继萍.膜分离与沉淀剂结合提取腌肠衣盐水中肝素纳的工艺研究[J].兰州医学院学报,2009,35(1):68-70.
作者姓名:李耀曾  曹芸  夏华  姚世霞  赵建邦  杜兴  张继萍
作者单位:李耀曾,曹芸,夏华,姚世霞,赵建邦,杜兴,LI Yao-zeng,CAO Yun,XIA Hua,YAO Shi-xia,ZHAO Jian-bang,Du Xing(甘肃省药品检验所,甘肃,兰州,730000);张继萍,ZHANG Ji-ping(陇盛肠衣厂,甘肃,陇西,748000)  
摘    要:目的探讨膜分离与沉淀剂结合提取腌肠衣盐水中肝素纳的工艺改进。方法采用盐解-离子交换-膜分离及蛋白沉淀除杂法从中提取肝素纳。结果从废弃的腌肠衣盐水中提取肝素钠取得了成功,与传统工艺(盐解-离子交换法)比较,洗脱液体积可浓缩至原体积的15%左右,乙醇用量可减少85%左右,而效价分别从传统工艺的96.82U/mg增加到157.58U/mg,总收率比传统工艺提高了12.5%。结论工艺方法可行、可靠,适宜于中、小型规模的生产。

关 键 词:腌肠衣盐水  膜分离  沉淀剂

Craft study Oil the extraction of heparin sodium from pickle casing brine by the combination of membrane separation and precipitator
LI Yao-zeng,CAO Yun,XIA Hua,YAO Shi-xia,ZHAO Jian-bang,Du Xing,ZHANG Ji-ping.Craft study Oil the extraction of heparin sodium from pickle casing brine by the combination of membrane separation and precipitator[J].Journal of Lanzhou Medical College,2009,35(1):68-70.
Authors:LI Yao-zeng  CAO Yun  XIA Hua  YAO Shi-xia  ZHAO Jian-bang  Du Xing  ZHANG Ji-ping
Institution:LI Yao-zeng, CAO Yun, XIA Hua, YAO Shi-xia, ZHAO Jian-bang, Du Xing, ZHANG Ji-ping (1. Gansu Provincial Institute for Drug Control, Lanzhou 730000, China; 2. Longsheng Heparin Sodium Factory, Longxi 748000, Gansu, China)
Abstract:Objective To investigate the craft of extracting heparin sodium from pickle casing brine by the combination of membrane separation and precipitator. Methods Heparin sodium was extracted by salt solution-ionic exchange-membrane filtration and the protein precipitation method. Results The extraction of heparin sodium from pickle casing brine abandoned was succeeded. When compared with traditional craft (salt solution-ionic exchange method), effluent volume could be condensed to 15% of the original physical volume, alcohol consumption could be reduced by 85% or so, but the titer was increased from 96.82 U/mg of traditional craft to 157.58 U/mg, and the total recovery rate was improved by 12.5%. Conclusion The method is feasible, the craft is credible and can be used for the production of small and medium-sized scales.
Keywords:pickle casing brine  membrane separation  precipitator
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