| 甘肃省半夏种质资源遗传多样性分析 |
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| 引用本文： | 张杰,徐涛,张冬梅,杨永建,陈集双.甘肃省半夏种质资源遗传多样性分析[J].兰州医学院学报,2007,33(2):38-44. |
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| 作者姓名： | 张杰 徐涛 张冬梅 杨永建 陈集双 |
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| 作者单位： | 浙江理工大学生物工程研究所，浙江理工大学生物工程研究所，兰州大学第一医院药剂科，兰州大学药学院，浙江理工大学生物工程研究所 浙江 杭州 310018，浙江 杭州 310018，甘肃 兰州 730000，甘肃 兰州 730000，浙江 杭州 310018 |
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| 摘 要： | 运用荧光标记引物的扩增片段长度多态性(AFLP)分子标记技术,对50份甘肃半夏样品进行了DNA指纹图谱构建和遗传差异分析。选出的8对AFLP引物共扩增出517条带,其中396条具有多态性,多态性位点百分率为75．8％,平均每对引物扩增出64．6条带和49．5个多态性条带。系统聚类分析结果表明所有半夏AFLP指纹均不相同,来源地相同的半夏表现出相对密切的亲缘关系,甘肃省西和县的半夏同天水市的半夏遗传差异明显。聚类分析结果获得了Bootstrap校验的支持。甘肃省半夏遗传多样性十分丰富,AFLP分子标记可有效应用于半夏种质资源鉴别和遗传多样性分析。
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| 关 键 词： | 半夏 扩增片段长度多态性 遗传多样性 DNA指纹图谱 |
| 文章编号： | 1000-2812（2007）02-0038-07 |
| 修稿时间： | 2007-01-09 |
AFLP analysis of genetic diversity of Pinellia ternata (Thunb.) Breit. in Gansu Province |
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| ZHANG Jie, Xu Tao, ZHANG Dong-mei, YANG Yong-jian, CHEN Ji-shuang.AFLP analysis of genetic diversity of Pinellia ternata (Thunb.) Breit. in Gansu Province[J].Journal of Lanzhou Medical College,2007,33(2):38-44. |
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| Authors: | ZHANG Jie Xu Tao ZHANG Dong-mei YANG Yong-jian CHEN Ji-shuang |
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| Institution: | 1. Institute of Bioengineering, Zhejiang Science and Technology University, Hangzhou 310018, China; 2. Department of Pharmacy, the First Hospital of Lanzhou University, Lanzhou 730000, China; 3. School of Pharmacy, Lanzhou University, Lanzhou 730000, China |
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| Abstract: | The DNA fingerprint and genetic variation of 50 individuals of Pinellia ternata (Thunb.) Breit from Gansu Province were analyzed by fluorescence labeled AFLP technique. A total of 517 DNA bands and 396 polymorphic DNA bands were detected by eight primer pairs screened from 64 primer pairs. The percentage of polymorphic bands was 75.8 %, total amplified bands and polymorphic bands per primer combination produced were 64.6 and 49.5, respectively, indicating a high level of genetic diversity existed in P. ternata in Gansu Province. The phylo-genetic cluster analysis revealed that all the individuals showed independent DNA fingerprints and could be absolutely differentiated in our assay. Individuals from similar regions were mainly clustered together and exhibited closer genetic relationship. Meanwhile, individuals from region of Longnan showed clear genetic distinction from those of Tianshui. The clustering results were strongly supported by Bootstrap testing. Our findings indicated that the genetic diversity of P. ternata was abundant in Gansu Province. AFLP markers can be potentially used as an effective tool for studying the genetic diversity and germplasm identification of P. ternata. |
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| Keywords: | Pinellia ternata (Thunb ) Breit amplified fragment length polymorphism genetic diversity DNA fingerprint |
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