2型糖尿病大鼠经不同干预后血浆内脏脂肪素水平的变化

目的探讨2型糖尿病大鼠经不同干预后血浆内脏脂肪素水平的变化及其与空腹血糖（FPG）、空腹胰岛素（FINS）、胰岛素抵抗指数（HOMA）、总胆固醇（TO）、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、游离脂肪酸（FFA）及体重的关系。方法采用高热量饲料加单次腹腔注射链脲佐菌素（35mg／kg）制备2型糖尿病大鼠模型，以1周后FPG≥11．1mmol／L为成模标准（n=28），将糖尿病大鼠分别予以瑞格列奈＋二甲双胍（B，n=7）、罗格列酮十二甲双胍（C，n=7）、胰岛素＋二甲双胍（D，n=7）、生理盐水（E，n=7）干预，8周末测定血生化指标并计算HOMA，采用ELISA法检测血浆内脏脂肪素水平。结果B—E组FPG、FINS、HOMA、TC、TG、LDL、FFA、内脏脂肪素及体重较对照组均显著升高（P〈0．01），HDL较对照组均明显下降（P〈0．01）；给予不同治疗后FPG、FINS、HOMA较未治疗组明显下降（P〈0．05），C组FFA较其他各组明显下降（P〈0．05），而内脏脂肪素较其他各组明显升高（P〈0．051；相关分析及多元回归分析显示血浆内脏脂肪素水平与体重及糖脂代谢相关。结论过氧化物酶体活化增殖受体（PPARr）核受体可能参与调控内脏脂肪素的表达和分泌活动；内脏脂肪素可能是PPARr的激动剂药物治疗2型糖尿病的作用靶点之一；内脏脂肪素与糖脂代谢和体重密切相关，与胰岛素抵抗无关。

Changes of plasma visfatin level in rats with type 2 diabetes after different interventions

Objective To investigate the change of plasma visfatin level and the relationship between plasma visfatin level with fasting plasma glucose （FPG）, fasting insulin （FINS）, homeostasis model assessment （HOMA）, total cholesterol （TC）, triglyceride （TG）, high density lipoprotein （HDL）, low density lipoprotein （LDL）, free fatty acids （FFA） and body weight to give different interventions in rats with type 2 diabetes. Methods With high-sucrose and high-fat diet＋STZ （30 mg/kg） inducing Wistar rats, rats model with type 2 diabetes melitus was developed by means of FPG〉11.1 mmol/L （DM, n=28）, and then given repaglinide＋dimethylbiguanide （B, n=7）,rosiglitazone＋dimethylbiguanide （C, n=7）, insulin＋dimethylbiguanide （D, n=7）, PS （E, n=7） in DM. Plasma visfatin levels were measured by ELISA in rats with type 2 diabetes and control after 8 weeks. The relationship of plasma visfatin level with FPG, FINS, HOMA, TC, TG, HDL, LDL, FFA and body weight were analyzed. Results FPG, FINS, HOMA, TC, TG, HDL, LDL, FFA, visfatin and body weight in diabetes rats and obese rats significantly increased and HDL significantly reduced compared with NC. FPG, FINS, HOMA in B-E groups significantly reduced compared with F. FFA in C group significantly reduced and visfatin level significantly increased compared with other groups. Correlation and multiple regression analysis showed that plasma visfatin level was closely correlated with glucolipid metabolism and body weight. Conclusion It is possible that peroxisome proliferator-activated receptors nuclear receptor joins in the express and secretory activity of visfatin. Visfatin, which is closely correlated with glucolipid metabolism and body weight, may be one of the targets of peroxisome proliferator-activated receptors excitomoter drug treat DM.