| 卡介苗ERP基因置换型打靶载体的构建 |
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| 引用本文： | 吴芳,张万江,曹旭东,黄春,吴江东.卡介苗ERP基因置换型打靶载体的构建[J].农垦医学,2005,27(5):332-334. |
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| 作者姓名： | 吴芳 张万江 曹旭东 黄春 吴江东 |
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| 作者单位： | 石河子大学新疆地方与民族高发病重点实验室,新疆,石河子,832002 |
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| 摘 要： | 目的：构建ERP基因打靶载体。方法：卡介前菌体外培养，扩增ERP基因两侧序列．连接载体与目的片段．筛选阳性克降并鉴定。结果：PCR扩增捕入片段大小与预期相符．鉴定证实PCR产物及插入片段为所需目的基因片段。结论：成功构建了用于卡介苗菌基因打靶的置换型载体，为今后构建ERP基因敲除株的卡介苗突变株的研究奠定基础。
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| 关 键 词： | 基因敲除 ERP基因 卡介苗菌 |
Construction of the gene targeting vector of ERP gene in BCG |
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| Wu Fang,Zhang Wanjiang,Cao Xudong,Huang Chun,Wu Jiangdong.Construction of the gene targeting vector of ERP gene in BCG[J].Agricultural Reclamation Medicine,2005,27(5):332-334. |
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| Authors: | Wu Fang Zhang Wanjiang Cao Xudong Huang Chun Wu Jiangdong |
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| Institution: | Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi University,Shihezi,Xinjiang 832002,China |
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| Abstract: | Objective: In order to knockout erp gene in BCG, the sideward gene of erp was amplified and gene targeting vector was constructed. Methods: Culturing the BCG in vitro, extracting the genome DNA, amplifying the targeted gene with PCR, constructing the gene targeting vector and identitying it. Results: The fragment was amplified successfully. The replacement vector with deleted erp gene was constructed. Conclusion: Constructing successfully the replacement vector which is used to the gene knockout in BCG. |
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| Keywords: | Gene knockout erp gone BCG |
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