宁夏2005年部分地区麻疹野病毒分离及基因型分析

目的了解2005年宁夏麻疹流行株基因型特征,为进一步的麻疹防治策略提供科学依据。方法采集宁夏2005年麻疹暴发病例的咽拭子或尿液标本19份,用EB病毒转化的狨猴淋巴母细胞(B95a)分离麻疹病毒。使用逆转录-聚合酶链反应(RT-PCR)方法扩增出分离的麻疹病毒株核蛋白(N)基因C末端676个核苷酸。再对扩增产物进行核苷酸序列测定和分析,并以C末端456个核苷酸片断构建基因亲缘性关系树,进行核苷酸同源性分析。结果从19份标本中分离到13株麻疹病毒,均为H1基因型H1a亚型。结论引起2005年宁夏部分地区麻疹暴发流行的野毒株为H1基因型,并以H1a为绝对优势亚型。不同暴发之间存在相同毒株引起的传播链,同一暴发中存在不同毒株的传播。

Isolation and genotyping of wild-type measles viruses in part region of Ningxia in 2005

Objective To learn genotype characterization of measles viruses in Ningxia in 2005 and provide scientific evidences for further strategy of controlling and preventing measles.Methods 19 throat swabs or urine specimens collected from measles patients broke out in Ningxia in 2005 were isolated viruses by using B95a cell line.The 676 nucleotides sequence of the C terminus of nucleoprotein(N)gene of isolates were amplified by RT-PCR,and the amplicons were directly sequenced.The phylogenetic tree based on the 456 C terminus nucleotide sequence of N gene was constructed and analyzed.Results 13 measles viruses were isolated from 19 specimens.Al1 of the isolates belonged to Hl genotype and H1a subgenotype.Conclusion It was H1 genotype measles viruses that caused measles outbreak in part region of Ningxia,and H1a subgenotype was the predominant virus.The same wild-type MV strain could induce outbreaks in different areas,and different MV strains had been transmitting in one outbreak.