荧光定量-PCR检测乙肝患者血清HBV-DNA及其临床应用

目的　探讨HBV -DNA基因含量与HBV -M两者的关系及其在临床中的应用。方法　采用酶联免疫吸附试验 (ELISA)和荧光定量 -PCR(FQ -PCR)法对 5 5 3例乙肝患者及无症状携带者血清进行HBV -M以及HBV -DNA定量检测。结果　乙肝患者血清HBV -DNA阳性率和基因含量与HBV -M模式有很大关系 ,与HBeAg(+)高度相关 ,显著高于其他各组 (P <0 .0 0 1,P <0 .0 1) ,在抗 -HBe(+)组或抗 -HBs(+)组也检出了一定浓度的HBV -DNA ;不同临床类型乙型肝炎患者血清中HBV -DNA的阳性率和含量无显著性差异 (P >0 .0 5 )。结论　单凭血清免疫标志物模式难以准确判断HBV的复制程度及传染性的强弱 ,定量检测HBV -DNA能真实反映HBV的复制情况 ,对诊断、治疗乙肝患者及疗效观察具有较大的指导意义。

The clinical significance of detecting HBV-DNA in the serum of HBV infectors by FQ-PCR

Objective To analyses the relationship an d clinical significance between the hepatitis B virus DNA (HBV-DNA) and HBV-M cont ent.Methods The amount of HBV-DNA in the serum of 553 patients with hepatiti s 13 was measured with ELISA and FQ-PCR.Results There was a good relationship between HBV-DNA positive rate gene content and HBV-M model in hepatitis B patients. The level of HBV-DNA in those patients was significantl y higher than other groups (P<0.001 or P<0.01) and there were also som e level of HBV-DNA in anti-HBe positive group and anti-HBs positive group .But t here was no significance in HBV-DNA positive rate and content in different clini cal types of HBV infected patients.Conclusion It was inac curate to evaluate the degree of HBV replication as well as infected ability on ly by serum immune. Instead the level of HBV-DNA can reflect the HBV replicatio n truly and there was also a great significance to diagnose. Treat and observe H BV patients.