自发性高血压大鼠脂联素及其受体 1 的表达与心血管重构的关系

目的观察自发性高血压大鼠（SHR）血清脂联素（APN），心肌、主动脉脂联素受体1（AdipoRl）的表达，探讨三者与血压升高、心血管重构的关系。方法选取22周龄SHR和Wistar—Kyoto（WKY）大鼠各8只为研究对象，测定血清APN、血清空腹胰岛素（FINS），实时荧光定量PCR法测定心肌、主动脉AdipoRlmRNA表达。结果与同周龄WKY组比较，SHR组收缩压升高，IR程度增加，心脏重量指数（HWI）和左心室重量指数（LVwI）增加，主动脉中膜（MT）增厚、壁腔比值（MT／LD）增加，血清APN水平、心肌、主动脉AdipoRlmRNA表达下降（P〈0．05），血清APN（X1）、主动脉AdipoRlmRNA（X2）、HOME—IR（X3）、SBP（x4）与MT／LD有关。结论SHR存在显著的心血管重构，APN及AdipoR1表达下降、IR程度增加；APN及AdipoRl可能是预防高血压及高血压心血管重构的重保护因素。

Relationship between expression of adiponectin including its receptor 1 and ventricular remodeling in spontaneously hypertensive rats

Objective To observe the expression of the serum adiponectin hormone （APN）, myocardial and aortic adiponectin receptor 1 （AdipoR1） in spontaneously hypertensive rats （SHR） in order to explore the relationship between the three aspects and ele- vation of blood pressure and cardiovascular remodeling. Methods Eight （22 weeks old） rats of SHR and WKY respectively as objects of study and to measure its APN, and FINS. The mRNA expression of AdipoR1 in myocardium and aortic was measured by actual time PCR. Results Compared with the same age group rats of WKY group, the systolic pressure, the degree of IR, HWI, LVWI, MT and MT/LD of SHR group were increased. The APN level of blood serum and the expression of protein and mRNA of myocardial and aortic AdipoR1 were decreased （P 〈 0.05）. Conclusion The rats of SHR group exhibit obvious cardiovascular remodeling. The expression of APN of blood serum and the expression of myocardial and aortic AdipoR1 are decreased. The degree of IR is increased. APN and Adi- poRl may be the important protective factor for hypertension and cardiovascular remodeling.Objective To observe the expression of the serum adiponectin hormone （APN）, myocardial and aortic adiponectin receptor 1 （AdipoR1） in spontaneously hypertensive rats （SHR） in order to explore the relationship between the three aspects and ele- vation of blood pressure and cardiovascular remodeling. Methods Eight （22 weeks old） rats of SHR and WKY respectively as objects of study and to measure its APN, and FINS. The mRNA expression of AdipoR1 in myocardium and aortic was measured by actual time PCR. Results Compared with the same age group rats of WKY group, the systolic pressure, the degree of IR, HWI, LVWI, MT and MT/LD of SHR group were increased. The APN level of blood serum and the expression of protein and mRNA of myocardial and aortic AdipoR1 were decreased （P 〈 0.05）. Conclusion The rats of SHR group exhibit obvious cardiovascular remodeling. The expression of APN of blood serum and the expression of myocardial and aortic AdipoR1 are decreased. The degree of IR is increased. APN and Adi- poRl may be the important protective factor for hypertension and cardiovascular remodeling.