| CIK、DC-CIK细胞杀伤胃癌MKN-28细胞的作用比较 |
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| 引用本文： | 张成磊,陈耀平,杨宝珍,潘月英,王妍柏.CIK、DC-CIK细胞杀伤胃癌MKN-28细胞的作用比较[J].宁夏医学杂志,2012,34(1):7-9,1. |
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| 作者姓名： | 张成磊 陈耀平 杨宝珍 潘月英 王妍柏 |
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| 作者单位： | 1. 宁夏医科大学医学实验中心,宁夏,银川,750004
2. 宁夏医科大学总医院检验科,宁夏,银川,750004 |
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| 基金项目： | 宁夏自然科学基金项目(NZ09111) |
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| 摘 要： | 目的研究细胞因子诱导的杀伤细胞(CIK)与树突状细胞(DC)共培养后对胃癌MKN-28细胞株的杀伤作用。方法取正常人外周血,分离出外周血单个核细胞(PBMC),加入不同的细胞因子分别诱导出DC和CIK细胞,共培养成DC-CIK细胞,以MTS法测定不同组CIK细胞对MKN-28杀伤活性。结果与单纯CIK细胞相比较,DC-CIK组细胞增殖速率明显提高(P<0.05);具有更强的杀伤胃癌MKN-28细胞株的活性(P<0.05),效靶比为20.0∶1时,DC-CIK组对MKN-28的杀伤活性为(38.02±2.06)%,>CIK组的(29.78±1.84)%,差异有统计学意义。结论 DC-CIK细胞是一种增殖活性和细胞毒作用均高于单纯CIK细胞的免疫活性细胞。
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| 关 键 词： | 细胞因子诱导的杀伤细胞 树突状细胞 胃癌 MKN-28 |
Comparison of the killing effect of CIK cells and DC-CIK cells of gastric MKN-28 cells |
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| Institution: | ZHANG Cheng-lei,et al. Clinical Laboratory,Ningxia Medical University,Yinchuan 750004,China |
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| Abstract: | Objective To study the killing effect of gastric MKN-28 cells of cytokine-induced killer cells(CIK) co-cultured with dendritic cells(DC).Methods Peripheral blood mononuclear cells(PBMC) were isolated from healthy human,DC and CIK cells were induced by different cytokines,and they were co-cultured to produce DC-CIK cells.The killing activity of different CIK groups against MKN-28 was determined by MTS.Results Compared with CIK,the DC-CIK presented a significantly higher proliferation(P<0.05).The killing effects of gastric MKN-28 cells were enhanced(P<0.05).The activity of cytotoxicity to MKN-28 in DC-CIK group was 38.02%±2.06%,and it was stronger than that in CIK group(29.78±1.84%),respectively(E/T=20:1).Conclusion DC-CIK cell is a kind of immune cell which has a higher proliferation and cytotoxicity than that in CIK cell. |
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| Keywords: | Cytokine-induced killer cells Dendritic cells Gastric MKN-28 |
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