荧光定量PCR检测结核分枝杆菌

目的比较荧光定量PCR与抗酸染色、结核抗体检测结核分枝杆菌的灵敏度和特异性,评价荧光定量PCR检测临床标本中结核杆菌的应用价值。方法对48例临床确诊的结核病患者和21例非结核呼吸系统疾病患者的痰标本应用荧光定量PCR法和痰涂片抗酸染色法检测,并分离患者外周血血清检测结核抗体,对3种检测方法的结果进行分析比较。结果抗酸染色法检测结核杆菌阳性率为22.9%,特异性为100%;血清结核抗体检测阳性率为77.1%特异性为85.7%;荧光定量PCR检测阳性率为52.1%,特异性为100%。结论荧光定量PCR是一种快速、防污染、敏感性高、特异性强的结核分枝杆菌辅助诊断方法,在临床上有重要应用价值。

Fluorescence quantitative PCR detection mycobacterium tuberculosis

Objective To compare the sensitivity and specificity of the there methods i.e.acid-fast stain,serum tuberculosis antibody and fluorescence quantitative PCR.Evaluate the application value of fluorescence quantitative PCR in detection clinical mycobacterium tuberculosis.Methods The specimens of sputum from 48 tuberculosis patients and 21 patients with non-tuberculosis respiratory system disease,M.tuberculosis were detected by acid-fast stain,fluorescence quantitative PCR and the serum tuberculosis antibody respectively.Finally we analyze and compare the results of the there methods.Results The positive rates of acid-fast stain,tuberculosis antibody and fluorescence quantitative PCR were 22.9%,77.1% and 52.1% respectively.The specificity of acid-fast stain,tuberculosis antibody and fluorescence quantitative PCR were100%,85.7% and 100% respectively.Conclusion Fluorescence quantitative PCR is a rapid and contamination-proof method for diagnosis tuberculosis,which shows that high specificity and sensitivity.It is a useful tool for clinic.