超氧化物歧化酶、阿魏酸钠抗肝缺血再灌注损伤的对比研究

目的：比较外源性超氧化物歧化酶（ＳＯＤ）与阿魏酸钠（ＳＦ）对肝缺血再灌注损伤的保护作用。方法：将２４只成年雄性ＳＤ大鼠随机分为对照组、ＳＦ保护组和ＳＯＤ保护组。通过阻断大鼠肝门１ｈ后再开放建立肝缺血再灌注损伤模型，在肝缺血前及肝再灌注２ｈ时测肝组织丙二醛（ＭＤＡ）、ＳＯＤ和测血ＡＬＴ、ＡＳＴ。并取肝组织作光镜及电镜观察。结果：再灌注２ｈ时ＳＦ保护肝组织内ＳＯＤ消耗明显多于ＳＯＤ保护组（Ｐ＜０．０１），肝组织内ＭＤＡ生成亦明显多于ＳＯＤ保护组（Ｐ＜０．０５）。两组肝细胞的显微、超微结构的改变基本一致。结论：ＳＦ清除氧自由基（ＯＦＲ）的作用逊色于ＳＯＤ，但两者对鼠肝缺血再灌注所致肝细胞的结构和功能损伤的保护作用基本相同

A Comparison of the Effect of Superoxide Dismistase and Sodium Ferulate on Damage of Ischemia Reperfusion in Rat Liver

To compare the effect of the damage of ischemia reperfusion in rat liver among superoxide dismistase (SOD), and sodium ferulate (SF) protection groups and control group, the animal model of hepatic ischemia was made by temporary blocking the blood vessels in hepatic portal and releasing the hepatic portal occlusion one hour later. The malondialdehyde (MDA), SOD in liver tissue, AST, ALT in blood were measured before the vessels were blocked and 2 hours after the liver was reperfusion. The hepatic tissue was examined under light and electronic microscopic. Result: lncreasing of MDA and decreasing of SOD in SF protection group (P<0.01, P<0.05),AST, ALT and the changes of the liver tissue under light and electronic microscop in two groups had no difference. Conclusion: The ability to scavenge oxygen free radical (OFR) of SOD is more obvious than that of SF, but the effect on the damage of ischemia reperfusion in rat liver for two groups has no difference.