| GFP-HPV16 E5融合蛋白在HeLa细胞内的表达与定位 |
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| 引用本文： | 唐双阳,陈杰,李薇,周良,余敏君,朱翠明,万艳平.GFP-HPV16 E5融合蛋白在HeLa细胞内的表达与定位[J].南华大学学报(医学版),2010,38(6). |
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| 作者姓名： | 唐双阳 陈杰 李薇 周良 余敏君 朱翠明 万艳平 |
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| 作者单位： | 南华大学病原生物学研究所; |
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| 摘 要： | 目的 研究GFP-HPV16 E5融合蛋白在HeLa细胞内的表达与定位.方法 PCR扩增HPV16E5基因片段,将其连接到真核表达截体pEGFP-C1,双酶切及测序鉴定,构建表达截体pEGFP-C1/HPV16 E5;将质粒pEGFP-C1/HPV16 E5和pEGFP-C1分别转染HeLa细胞,在倒置荧光显微镜下现察GFP-HPV16 E5融合蛋白在HeLa细胞内的表达与定位.结果 GFP 荧光均匀分布在细胞浆和细胞核;GFP-HPV16 E5融合蛋白组荧光分布在细胞浆.结论 GFP-HPV16 E5融合蛋白能在HeLa细胞内表达且定位于细胞浆.
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| 关 键 词： | 人乳头瘤病毒16型 E5 定位 |
Expression and Localization of GFP-HPV16 E5 Fusion Protein in HeLa Cells |
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| TANG Shuang-yang.CHEN Jie,LI Wei,et al.Expression and Localization of GFP-HPV16 E5 Fusion Protein in HeLa Cells[J].Journal of Nanhua University(Medical Edition),2010,38(6). |
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| Authors: | TANG Shuang-yangCHEN Jie LI Wei |
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| Institution: | TANG Shuang-yang.CHEN Jie,LI Wei,et al (Institute of Pathogenic Biology,University of South China,Hengyang,Hunan 421001,China) |
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| Abstract: | Objective To investigate the expression and localization of GFP - HPV16 E5 fusion proteins in HeLa cells.Methods HPV16 E5 gene fragment amplified by PCR was inserted into eukaryotic expression vector of pEGFP -C1 with the same restriction sites.Enzymes digestion and DNA sequencing were used to identify E5 gene fragment.The eukaryotic expression vector of pEGFP - C1/HPV16 E5 or pEGFP - C1 was transfected into HeLa cells,respectively.The expression and localization of GFP - HPV16 E5 or GFP was observed under ... |
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| Keywords: | HPV16 E5 localization |
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