Caveolin-1基因转染对人胃腺癌细胞生长的影响

目的 探讨外源性Caveolin-1基因在人胃腺癌细胞中的表达作用.方法 将pcl-neo-caveolin-1人全长质粒转染入MGC803细胞中,用G418筛选得到细胞克隆并扩大培养,获得稳定表达caveolin-1的细胞系,通过Western-blot、细胞计数及流式细胞术等方法检测基因表达、细胞增殖分化等情况.结果 与对照组和pcl-neo空质粒转染组相比,转染caveolin-1基因后的MGC803细胞的caveolin-1的表达明显增高,而P-ERK1/2的表达却明显下降;细胞的群体倍增时间明显延长:由46.67或47.32小时延长至65.46小时,差异有显著性(P＜0.01); 细胞周期分布发生明显变化:G0/G1期细胞比例由35.02%增加到51.98%,S期比例由56.97%下降至39.80%,G2、M期无明显变化.结论 Caveolin-1通过抑制MGC803细胞的增殖而导致其发生G0/G1期阻滞,可为肿瘤基因治疗提供实验依据.

Effects of Recombinant Caveolin-1 Gene Transduction on Proliferation of Human Gastric Adenocarcinoma Cells

Objective To explore the role of caveolin- 1 gene in human gastric adenocarcinoma cells. Methods The polneo- caveolin- 1 gene, a human full long gene was transfected into the MGC803, a kind human gastric mucoid adenocarcinoma cell line, then a new cell line which could stably express caveolin- 1 was gained. Westernblot, cell counting and flow cytometry were used to detect protein expression, cell proliferation, cell differentiation,and so on. Results The expression of caveolin - 1 in MGC803 cells which transfected with caveolin - 1 was significantly up - regulated, while P- ERK1/2 was significantly down - regulated. MGC803 cells which transfected with caveolin- 1 have an extended doubhng time, and the cell proliferation were inhibited, the cells were induced to exit S phase of cell cycle with an increase in the G0/G1 population. Conclusions Caveolin - 1 could inhibit the cell proliferation with an arrest in G0/G1 phase which was connected with the activeation of ERK.