首页 | 本学科首页   官方微博 | 高级检索  
检索        

合并前列腺炎的良性前列腺增生组织中Ki-67,Bcl-2,Bax和caspase-3表达及意义
引用本文:王龙,杨金瑞,杨罗艳,刘紫庭,饶建明,刘龙飞.合并前列腺炎的良性前列腺增生组织中Ki-67,Bcl-2,Bax和caspase-3表达及意义[J].中南大学学报(医学版),2008,33(3):222-226.
作者姓名:王龙  杨金瑞  杨罗艳  刘紫庭  饶建明  刘龙飞
作者单位:中南大学湘雅二医院泌尿外科, 长沙 410011
基金项目:湖南省科技计划项目(06SK3035)
摘    要:目的:通过检测单纯良性前列腺增生(benign prostatic hyperplasia,BPH)与合并前列腺炎的BPH患者中Ki-67,Bcl-2,Bax和caspase-3蛋白表达差异,探讨炎症与BPH发生发展的关系.方法:收集58例行经膀胱前列腺摘除手术的BPH患者的前列腺标本,常规HE染色后观察其合并前列腺炎情况,其中单纯BPH 16例,合并前列腺炎的BPH 42例.采用免疫组化和Western免疫印迹技术研究Ki-67,Bcl-2,Bax和caspase-3在前列腺组织中的表达情况.结果:合并前列腺炎的BPH患者Ki-67和Bcl-2表达有显著升高(P<0.05),caspase-3表达显著下调(P<0.05),但Bax的表达无明显变化(P>0.05).结论:前列腺炎促进BPH细胞Ki-67和Bcl-2蛋白表达,减少caspase-3蛋白激活,提示炎症可能参与了BPH的发生发展过程.

关 键 词:良性前列腺增生  前列腺炎  Ki-67  Bcl-2  caspase-3  
文章编号:1672-7347(2008)03-0222-05
收稿时间:2007-7-25
修稿时间:2007年7月25日

Expression of Ki-67,Bcl-2,Bax and caspase-3 in benign prostatic hyperplasia combined with prostatitis and their significances
WANG Long,YANG Jin-rui,YANG Luo-yan,LIU Zi-ting,RAO Jian-ming,LIU Long-fei.Expression of Ki-67,Bcl-2,Bax and caspase-3 in benign prostatic hyperplasia combined with prostatitis and their significances[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2008,33(3):222-226.
Authors:WANG Long  YANG Jin-rui  YANG Luo-yan  LIU Zi-ting  RAO Jian-ming  LIU Long-fei
Institution:Department of Urology, Second Xiangya Hospital, Central South University, Changsha 410011, China
Abstract:OBJECTIVE: To detect the expression of Ki-67, Bcl-2, Bax and caspase-3 in simple benign prostatic hyperplasia (BPH) and BPH combined with prostatitis,and to evaluate the effect of inflammation on the development and progression of BPH. METHODS: All specimens were obtained from patients undergoing surgical resection of the prostate. The paraffin section of the specimens was stained with hemotoxyline and eosin, and observed under light microscope to examine the inflammation hispathological changes. Sixteen patients with simple BPH (Group A) and 42 patients with BPH combined with prostatitis (Group B) were included. Immunohistochemical analysis and Western blot were used to examine the expression of Ki-67, Bcl-2, Bax and caspase-3. RESULTS: The expression of Ki-67 and Bcl-2 was significantly higher in Group B than that in Group A (P<0.05), and caspase-3 expression was significantly lower (P<0.05). There was no difference in Bax expression between the 2 groups (P>0.05). CONCLUSION: Prostatitis can up-regulate Ki-67, Bcl-2 expression, and down-regulate the expression of caspase-3 in BPH. Prostatitis appeared to play an important role in the development of BPH by affecting the proliferation and apoptosis of the prostatic cells.
Keywords:benign prostatic hyperplasia  prostatitis  Ki-67  Bcl-2  caspase-3
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《中南大学学报(医学版)》浏览原始摘要信息
点击此处可从《中南大学学报(医学版)》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号