脑溢安颗粒对脑出血大鼠脑内IL-6mRNA及蛋白表达的影响

目的：探讨脑溢安颗粒 (简称脑溢安)对脑出血的保护作用机制。方法：采用胶原酶注射建立大鼠脑出血模型，以原位杂交及Western blotting方法检测实验性脑出血大鼠脑内白介素6（interleukin-6，IL-6）mRNA与蛋白的表达及脑溢安的影响。结果：脑溢安治疗组脑内IL-6 mRNA表达信号在术后4~7 d较模型组增强，且IL-6蛋白表达在术后6 h~7 d均明显高于模型组(P<0.01)。 结论：上调IL-6蛋白的表达、维持IL-6 mRNA及蛋白的高水平表达可能是脑溢安保护脑出血损伤的作用机制之一。

Effects of Naoyian granule on the expression of IL-6 mRNA and protein in the experimental intracerebral hemorrhagic brain in rats

OBJECTIVE: To explore the protective mechanisms of the traditional Chinese medicine complex Naoyian granule (NYA) on intracerebral hemorrhagic (ICH). METHODS: ICH rat models were established by stereotaxically injecting 0.4 U type VII collagenase into the right globus pallidus (GP), the expression of IL-6mRNA and protein were investigated by in situ hybridization and Western blotting. RESULTS: In the NYA group the IL-6 mRNA level was higher than that of the model group on 4 - 7 d and the IL-6 protein level was remarkably higher than that of the model group on 6 h - 7 d after ICH injury (P <0.01). CONCLUSION: Up-regulating the expression of IL-6 protein, and stabilizing the increasing expression of IL-6 mRNA and protein may be the protective mechanisms of NYA.