经鼠中心静脉途径注射重组腺病毒的基因转染效率及靶向性

目的：探讨经中心静脉途径注射外源基因在大鼠体内不同组织的表达及靶向性。 方法:将重组腺病毒AdLacZ经中心静脉途径导入SD大鼠体内，以X-gal染色法检测腺病毒介导的标识基因（LacZ）在大鼠体内的表达部位和时间。结果:注射AdLacZ（1×109pfu/mL）后1d，大鼠肺、肝、肾、脾组织即有少量表达。3d后大鼠肺、肝、肾、脾组织LacZ基因表达明显，7d时达高峰，14d开始下降，28d基本消失。肺组织，第3,7,14,21d，LacZ基因明显高于其他组织（P<0.05）,脑组织始终未见表达。结论:腺病毒携带的外源基因，经中心静脉途径给药，可能是肺、肝、肾等疾病基因治疗的有效途径，尤其适用于肺部疾病的基因治疗。

Gene transfection efficiency and destination of recombinant adenovirus gene transfer through internal jugular vein injection in rats

OBJECTIVE: To investigate the adenovirus-mediated LacZ gene expression and the destination in different organs of SD rats after the intravenous injection in rats. METHODS: Recombinant adenovirus vector containing LacZ was transferred to SD rats by injecting into the internal jugular vein. To identify the sites and periods of LacZ gene expression, X-gal staining was used to detect beta-gal level and period of LacZ gene expression of different organs in the transfected and non-transfected rats at different time intervals. RESULTS: On the 1st day after the injection, the lung, liver, kidney, and spleen expressed some beta-gal; on the 3rd day after the injection, the lung, liver, kidney, and spleen expressed beta-gal obviously; their peak levels were on the 7th day; the beta-gal level decreased on the 14th day; beta-gal expression disappeared in the most organs except the lungs on the 28th day. In all animals, the brain did not express any beta-gal. CONCLUSION: The adenovirus-mediated exogenous gene transfer in the internal jugular vein may be an effective approach of gene therapy in some diseases in the lung, liver, and kidney.