| 结核杆菌异烟肼耐药性产生的分子机制研究 |
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| 引用本文： | 成光杰,徐海明,谢慎思,彭兴华,朱定尔.结核杆菌异烟肼耐药性产生的分子机制研究[J].中南大学学报(医学版),1996(6). |
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| 作者姓名： | 成光杰 徐海明 谢慎思 彭兴华 朱定尔 |
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| 作者单位： | Research Genter of Molecular Biology，Hunan Medical University |
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| 摘 要： | 本文设计了两对引物，分别采用ＰＣＲ扩增结核杆菌异烟肼耐药性相关基因—ＫａｔＧ基因酶活性中心区，并用单链构象多态性分析（ＳＳＣＰ）及ＤＮＡ序列分析方法对临床分离的９株异烟肼敏感株、１１株异烟肼中、低度耐药株进行了耐药性分子机制的研究，结果发现中国人结核杆菌产生异烟肼耐药性的主要原因是由于ｋａｔＧ基因发生碱基点突变所致，本资料亦为快速检测结核杆菌异烟肼耐药性研究方法提供资料。
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| 关 键 词： | 结核杆菌 耐药性 多聚酶链反应 单链构象多态性 基因，触酶—过氧化物酶 |
STUDIES ON MOLECULAR MECHANISM OF ISONIAZID RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS |
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| Cheng Guangjie,Xu Haiming,Xie Shensi,Pen Xinghua,Zhu Ding-er.STUDIES ON MOLECULAR MECHANISM OF ISONIAZID RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1996(6). |
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| Authors: | Cheng Guangjie Xu Haiming Xie Shensi Pen Xinghua Zhu Ding-er |
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| Abstract: | wo pairs of primers were designed.Nine isolates of isoniazid-sensitive and 11 isolates ofisoniazid-resistant M.tuberculosis were analyzed by polymerase chain reaction amplification of396bp sequence of katG gene.With the use of single-strand conformation polymorphism analysisand DNA sequencing,8 isolates in 396bp products and 5 isolates in 2l6bp products of the ll iso-lates showed point mutations and no deletion or insertion was detected.Thus,in China,pointmutations in katG gene can mainly account for inactivation of catalase-peroxidase,leading to iso-niazid resistance.The results provide laboratory evidence for detecting the isoniazid resistance in M.tuberculosis and also explore its molecular mechanism. |
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| Keywords: | mycobacterium tuberculosis drug resistance polymerase chain reaction SSCP gene catalase-peroxidase |
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