光氧化反应处理牛颈静脉带瓣管道的形态学与理化性能研究

目的：探讨光氧化反应处理后的牛颈静脉带瓣管道的外观形态、组织稳定性及流体物理性能的特征。方法：取新鲜牛颈静脉带瓣管道24根，分为光氧化处理组、戊二醛处理组及新鲜对照组。分别观察和测量各组处理前后的管壁的厚度、管腔外径及各组管道瓣膜的抗返流功能的变化，测定处理前后的组织含水量、热皱缩温度、最大应力，并作各组的组织蛋白提取分析以了解组织稳定性。结果：光氧化组与对照组相比管壁厚度、管腔外径、瓣膜抗返流功能、组织含水量及热皱缩温度差异无统计学意义（P>0.05）,与戊二醛组相比差异有统计学意义（P<0.05）。光氧化组最大应力大于对照组，低于戊二醛组。光氧化组和戊二醛组的组织蛋白提取量均少于对照组。结论：光氧化方法处理后的牛颈静脉带瓣管道能较好地保持原有的管壁及瓣膜结构的完整性，并可提高组织的稳定性和组织的断裂强度。

Morphologic and physiochemical properties of bovine jugular conduit stabilized by dye-mediated photo-oxidation

OBJECTIVE: To determine the morphologic and physiochemical properties of bovine jugular conduit with valves stabilized by dye-mediated photo-oxidation. METHODS: Twenty-four bovine jugular conduits with valves were divided into 3 groups and treated with dye-mediated photo-oxidation (Group I), glutaraldehyde (Group II) and untreated group (Group II), respectively. Morphologic and physiochemical properties of the 3 groups, including wall thickness, diameter, tissue water content, heat shrinking temperature, breaching strength, and tissue protein extraction assay were studied. RESULTS: There was no difference in wall thickness, diameter, tissue water content, and heat shrinking temperature between Group I and II ,but there was significant difference between Group I and II. The breaching strength of Group I was higher than that of Group IU (P < 0.05), but lower than that of Group II (P < 0. 05). A decrease in extractable tissue protein was found in Group I and II. CONCLUSION: The dye-mediated photooxidation can effectively preserve the structure and the anti-regurgitation function of valves and improve the tissue stability and enhance the tension of bovine jugular conduit with valves.