| 人类巨细胞病毒感染对神经胶质瘤细胞HOXB5,HOXB6,HOXB7及HOXB8基因表达的影响 |
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| 引用本文： | 邬国军,陈利玉,戴橄,姚孟辉,陈淑贞,舒明星,罗敏华.人类巨细胞病毒感染对神经胶质瘤细胞HOXB5,HOXB6,HOXB7及HOXB8基因表达的影响[J].中南大学学报(医学版),2001,26(5):409-411. |
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| 作者姓名： | 邬国军 陈利玉 戴橄 姚孟辉 陈淑贞 舒明星 罗敏华 |
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| 作者单位： | 中南大学湘雅医学院微生物学教研室 |
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| 基金项目： | 国家自然科学基金资助 ( 3970 0 0 0 6 ) |
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| 摘 要： | 以半定量RT -PCR技术检测经人类巨细胞病毒 (HCMV)感染和 /或全反式维甲酸 (ATRA)处理的神经胶质瘤细胞U2 5 1株的HOXB5 ,HOXB6 ,HOXB7和HOXB8的相对表达水平 ,以探讨HCMV致畸的可能机制。结果示 ,未经处理的U2 5 1细胞不表达HOXB5 ,HOXB6及HOXB8,但表达HOXB7;HCMV感染和 /或ATRA处理后 ,细胞仍不表达HOXB5及HOXB6 ,但在第二代时均表达HOXB8,尤以HCMV和ATRA共同处理后更明显 ;第三代后HOXB8的表达恢复正常。HCMV和 /或ATRA处理细胞后 ,HOXB7的表达在第一代即上调 ,第二代、第三代其表达稍下降 ,至第四代时表达又上调。表明HCMV致畸作用可能是通过诱导HOX基因的表达进而调节其它基因的异常表达而实现。
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| 关 键 词： | HOX基因 人类巨细胞病毒 RT-PCR 神经胶质瘤 细胞 |
| 文章编号： | 1000-5625(2001)-05-0409-03 |
| 修稿时间： | 2001年1月3日 |
Influence of human cytomegalovirus infection on the expression of HOXB5,HOXB6,HOXB7,and HOXB8 genes in gliomaous cells |
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| WU Guo jun,CHEN Li yu,DAI Gan,et al.Influence of human cytomegalovirus infection on the expression of HOXB5,HOXB6,HOXB7,and HOXB8 genes in gliomaous cells[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2001,26(5):409-411. |
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| Authors: | WU Guo jun CHEN Li yu DAI Gan |
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| Institution: | (Department of Microbiology,Xiangya School of Medicine,Central South University,Changsha 410078, China) |
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| Abstract: | Objective The expressions of HOXB5,HOXB6,HOXB7,and HOXB8 genes of U251 cell infected by human cytomegalovirus and/or treated with all trans-retinoic acid(ATRA) were detected by semi-quantitative RT-PCR.The results were that U251 cell did not express HOXB5,HOXB6, and HOXB8 but expressed HOXB7 without infecting HCMV and/or being treated with ATRA.After infected with HCMV and/or treated with ATRA,the expressions of HOXB7 and HOXB8 were up-regulated and the expression of HOXB7 lasted the fourth generation,while HOXB8 was up-regulated only in the second generation.These results indicate that the abnormal expression of HOXB gene induced by HCMV may play an important role in the maldevelopment. |
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| Keywords: | HOX genes human cytomegavovirus RT-PCR glioma cells |
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