首页 | 本学科首页   官方微博 | 高级检索  
检索        

IGF-1对IL-1诱导的兔关节软骨细胞NO和PGE2的影响
引用本文:彭程,肖涛,罗远明,刘夏君,林绵辉,胡金玺.IGF-1对IL-1诱导的兔关节软骨细胞NO和PGE2的影响[J].中南大学学报(医学版),2008,33(3):197-203.
作者姓名:彭程  肖涛  罗远明  刘夏君  林绵辉  胡金玺
作者单位:1.中南大学湘雅二医院创伤骨科研究室, 长沙 410011; 2.上海市奉城医院骨科, 上海 201411
基金项目:国家自然科学基金(30571883)
摘    要:目的:检测合成性细胞因子胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)对损伤性细胞因子IL-1(interhukin-1)诱导的兔关节软骨细胞产生NO(nitric oxide)和前列腺素(prostaglandinE2,PGE2)的影响,探讨IGF-1在骨关节炎治疗中的作用机制.方法:实验分为IL-1β10μg/L组、IL-1β10 μg/L IGF-1 1μg/L组、IL-1β 10 μg/L IGF-1 10μg/L组、IL-1β 10 μg/L IGF-150 μg/L组、IL-1β 10 μg/L IGF-1 100 μg/L组、IGF-150 μg/L组和空白对照组.首先进行2月龄兔原代软骨细胞的培养并进行鉴定,然后将IL-1β 10 μg/L单独或与不同浓度的IGF-1共育于第2代兔关节软骨细胞,硝酸还原酶法测定实验组细胞上清液NO的含量,ELISA酶联免疫竞争法测定细胞上清液中PGE2含量,再对测定的NO和PGE<2的浓度与IGF-1和IL-1的浓度进行有关的统计学分析.结果:IL-1β10μg/L组NO浓度为(89.971±10.224) μmol/L,PGE2浓度为(22.028±8.731)ng/L;空白组NO浓度为(12.404±8.809)μmol/L,PGE<2浓度为(1.900±0.227)ng/L.IL-1β 10 μg/L组与空白组比较,NO和PGE<2明显增加,差异有统计学意义(P<0.05).在IL-1β均为10 μg/L时,IGF-1可以呈剂量依赖地降低IL-1诱导的兔关节软骨细胞NO和PGE2的升高,并且在50 μg/L时即可达到最佳浓度.结论:IL-1能增加软骨细胞培养中的NO和PGE2的产生.IGF-1在体外可以呈剂量依赖地降低IL-1诱导的兔关节软骨细胞NO和PGE2的升高,其最佳浓度为50 μg/L.

关 键 词:白介素-1  胰岛素样生长因子-1  一氧化氮  前列腺素E2  骨关节炎  软骨细胞  
文章编号:1672-7347(2008)03-0197-07
收稿时间:2007-4-16
修稿时间:2007年4月16日

Effect of IGF-1 on NO and PGE_2 in rabbit articular chondrocytes induced by IL-1
PENG Cheng,XIAO Tao,LUO Yuan-ming,LIU Xia-jun,LIN Mian-hui,HU Jin-xi.Effect of IGF-1 on NO and PGE_2 in rabbit articular chondrocytes induced by IL-1[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2008,33(3):197-203.
Authors:PENG Cheng  XIAO Tao  LUO Yuan-ming  LIU Xia-jun  LIN Mian-hui  HU Jin-xi
Institution:1.Research Institute of Traumatic Orthopaedics, Second Xiangya Hospital, Central South University, Changsha 410011;
2.Department of Orthopaedics, Fengcheng Hospital, Shanghai 201411, China
Abstract:OBJECTIVE: To explore the effect of insulin-like growth factor (IGF-1) on the concentration of NO and PGE(2) in the supernatant of rabbit articular chondrocytes induced by IL-1, and to explore the mechanism of IGF-1 in the development of osteoarthritis (OA). METHODS: The samples were divided into 7 groups: IL-1beta 10 microg/L group, IL-1beta 10 microg/L+IGF-1 1 microg/L group, IL-1beta 10 microg/L+IGF-1 10 microg/L group, IL-1beta 10 microg/L+IGF-1 50 microg/L group, IL-1beta 10 microg/L+IGF-1 100 microg/L group, IGF-1 50 microg/L group, and a blank control group. The chondrocytes from the articular cartilage of 2 month old rabbits were cultivated and identified, and then co-cultured in the second filial generation chondrocytes on plates with or without recombinant human IGF-1 or IL-1. The concentration of NO was detected by nitrate reductase kit, and that of PGE(2) by enzyme-linked immunosorbent assay (ELISA). The results were analyzed by statistical method. RESULTS: The average value of NO and PGE(2) was (89.971+/-10.224) micromol/L and (22.028+/-8.731) micromol/L in the IL-1beta 10 microg/L group, and (12.404+/-8.809) micromol/L and (1.900+/-0.227) ng/L in the blank control group. The concentration of NO and PGE(2) in IL-1beta 10 microg/L group was significantly higher than that in the blank control group (P<0.05). At the same concentration of 10 microg/L, IGF-1 could dose-dependently decrease the increase of NO and PGE(2) concentration induced by IL-1beta in the chondrocytes supernatant in vitro, and the optimum concentration of IGF-1 was 50 microg/L. CONCLUSION: IL-1 can significantly increase the concentration of NO and PGE(2), and IGF-1 can dose-dependently decrease the concentration of NO and PGE(2) in the chondrocytes supernatant in vitro. The optimum concentration of IGF-1 was 50 microg/L.
Keywords:interleukin-1  prostaglandin E2  nitric oxide  cytokines  osteoarthritis
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中南大学学报(医学版)》浏览原始摘要信息
点击此处可从《中南大学学报(医学版)》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号