氯胺酮诱导人膀胱SV-HUC-1 细胞凋亡的实验研究

目的：研究氯胺酮对人膀胱SV-HUC-1 细胞凋亡的影响，初步探讨氯胺酮相关性膀胱功能损伤的发 病机制。方法：培养人膀胱SV-HUC-1 永生化上皮细胞系，用不同质量浓度的氯胺酮作用于SV-HUC-1 细胞， 作用不同时间后用Annexin V-FITC/PI 双染法流式细胞术检测细胞凋亡率，用Western 印迹法检测Bax，Bcl-2， pro-caspase-3，cleaved caspase-3 蛋白表达的含量。结果：与对照组相比，加入氯胺酮后SV-HUC-1 细胞凋亡率增加， Bax 表达增高，Bcl-2 表达减弱，Bax 与Bcl-2 比值增高，pro-caspase-3 表达减少，cleaved caspase-3 表达增高(P<0.05)， 且与氯胺酮质量浓度和作用时间相关(P<0.05)。 结论：氯胺酮能够明显促进人膀胱SV-HUC-1 细胞的凋亡，并 呈浓度依赖性和时间依赖性。

Ketamine induces apoptosis of human uroepithelial SV-HUC-1 cells

Objective: To determine the effect of ketamine on the apoptosis of human uroepithelial cells (SVHUC- 1) and the pathogenesis of ketamine-associated cystitis. Methods: SV-HUC-1 cells were cultured under various concentrations of ketamine and different time. Flow cytometry was used to analyze the rate of cell apoptosis. The protein levels of Bax, Bcl-2, pro-caspase-3, and cleaved caspase-3 were detected by Western blot. Results: Compared with the control group, the apoptotic rate of ketamine cultured SV-HUC-1 cells increased. The expression of Bax increased, Bcl-2 expression decreased, and Bax/Bcl-2 in the ketamine cultured SV-HUC-1 cells was significantly higher. The protein level of pro-caspase-3 was significantly lower, and that of cleaved caspase-3 was significantly higher than that in the control group (P<0.05), positively correlated with the dose of ketamine and time of culture (P<0.05). Conclusion: Ketamine can induce the apoptosis of SV-HUC-1 cells in a dose and time dependent manner.