脐带间充质干细胞对卵巢癌细胞增殖和凋亡的影响

目的：观察脐带间充质干细胞(umbilical cord mesenchymal stem cells，UC-MSCs)抑制人卵巢癌SKOV3细胞增 殖及促进其凋亡的作用。方法：采用Transwell共培养观察UC-MSCs向卵巢癌细胞靶向归巢的作用；MTT 实验检测UCMSCs 条件培养基抑制SKOV3细胞增殖情况；Annexin V-FITC/PI双染法检测其凋亡率；实时PCR检测与SKOV3细胞增殖 和凋亡相关基因Ki-67，Bax，Bcl-2的表达。结果：UC-MSCs体外向SKOV3细胞靶向归巢。MTT 结果显示UC-MSCs条件 培养基明显抑制SKOV3细胞的增殖(P<0.01)。Annexin V-FITC/PI双染法提示75%条件培养基组的凋亡率明显高于对照 组(P<0.05)。实时PCR发现增殖相关基因Ki-67的表达明显下降(P<0.01)，凋亡相关基因Bcl-2的表达明显降低(P<0.01)， Bax的表达明显增加(P<0.01)。结论：UC-MSCs体外能向卵巢癌细胞靶向归巢，通过调节增殖相关基因Ki-67的表达， 抑制SKOV3细胞增殖；通过调节凋亡相关基因Bcl-2和Bax的表达，促进SKOV3细胞凋亡。

Effect of umbilical cord mesenchymal stem cells on proliferation and apoptosis of ovarian cancer cells

Objective: To observe the eff ects of human umbilical cord mesenchymal stem cells (UC-MSCs) on the proliferation and apoptosis of human ovarian cancer cell SKOV3. Methods: Transwell co-culture was used to observe the targeted homing eff ect of UC-MSCs on ovarian cancer cells. MTT assay was used to detect the inhibitory eff ect of UC-MSCs conditioned medium on SKOV3 proliferation, and Annexin V-FITC/PI double staining was used to detect the apoptotic rate. Real-time PCR was used to detect the expression levels of Ki-67, Bcl-2 and Bax genes-relevant to proliferation and apoptosis of SKOV3 cells. Results: UC-MSCs targeted SKOV3 cells in vitro. MTT assay showed that UC-MSCs conditioned medium significantly inhibited the proliferation of SKOV3 cells (P<0.01). Annexin V-FITC/ PI double staining showed that the apoptotic rate in the 75% conditioned medium group was significantly higher than that in the control group (P<0.05). Real-time PCR showed that the expression of proliferation-related gene Ki-67 decreased significantly (P<0.01). The apoptosisrelated gene Bcl-2 expression was decreased dramatically (P<0.01), and Bax expression was increased significantly (P<0.01). Conclusion: UC-MSCs can target ovarian cancer cells in vitro, inhibit the proliferation of SKOV3 cells by regulating the expression of Ki-67, and promote the apoptosis of SKOV3 cells by regulating the expression of Bcl-2 and Bax.