高血压肾损害患者血清IL-22水平变化及其临床意义

目的：通过观察原发性高血压及高血压肾损害患者血清白细胞介素-22(IL-22)浓度变化及与相关指标的关 系，探讨其在高血压肾损害中的作用及临床意义。方法：收集97例原发性高血压患者，根据24 h尿蛋白定量结果分为 单纯高血压组(n=45)和高血压肾损害组(n=52)，40例健康体检者为对照组。用酶联免疫吸附试验检测各组血清IL-22 水平，流式细胞术检测外周血Th22细胞亚群比例，免疫比浊法测定超敏C反应蛋白(hsCRP)的浓度，并进行相关性分 析。结果：与对照组相比，单纯高血压组血清IL-22水平升高，外周血Th22细胞比例明显上升(均P<0.01)；高血压肾 损害组血清IL-22较单纯单纯高血压组升高(P<0.01)，外周血Th22细胞亚群比例也高于单纯高血压组(P<0.05)。直线相 关分析显示：单纯高血压组血清IL-22与收缩压、舒张压及hsCRP水平呈正相关(r=0.367，P<0.01；r=0.402，P<0.01； r=0.329，P<0.05)，与Th 22细胞比例呈正相关(r=0.478，P<0.01)。高血压肾损害组血清IL-22水平与24 h尿蛋白定量呈正 相关(r=0.318，P<0.05)。结论：高血压肾损害患者血清IL-22水平及外周血Th 22细胞比例均较单纯高血压患者升高，血 清IL-22水平和血压水平与高血压肾损害的程度呈正相关，提示其可能参与高血压肾损害的发生发展。

Changes in serum IL-22 level in patients with hypertensive renal damage and its clinical significance

Objective: To observe the changes in serum interleukin-22 (IL-22) level and its correlation with other clinical indexes in patients with essential hypertension and hypertensive renal damage, and to investigate its eff ects and clinical signifi cance. Methods: A total of 97 essential hypertension (EH) patients and 40 healthy control were enrolled in this study. Th e EH patients were divided into a simple hypertension (SH) group (n=45) and a hypertensive renal damage (HRD) group (n=52) according to 24 h urinary protein (24 h-UPRO) level. Basic clinical data were collected; serum IL-22 level was detected by enzyme-linkedimmunosorbent assay (ELISA); the proportion of Th22 cells in peripheral blood was evaluated by flow cytometry (FCM); and serum high sensitivity C-reactive protein (hs-CRP) concentration was measured by immune turbidimetry. Correlation analysis was applied between serum IL-22 level and other indexes. Results: Serum IL-22 level and Th22 cell proportion in patients with hypertension were significantly higher than those in the healthy controls (all P<0.01). Compared with the SH group, serum IL-22 level and Th22 cell proportion were significantly elevated in the HRD group (P<0.01, P<0.05, respectively). Bivariate linear correlation analysis revealed that serum IL-22 level was positively correlated with systolic blood pressure (SBP), diastolic blood pressure (DBP), and hsCRP (r=0.367, P<0.01; r=0.402, P<0.01; r=0.329, P<0.05); there was also a positive correlation between serum IL-22 concentration and Th22 cell proportion (r=0.478, P<0.01) in the SH group. Serum IL-22 level was positively correlated with 24 h-UPRO level (r=0.318, P<0.05) in the HRD group. Conclusion: Serum IL-22 level and peripheral blood Th22 cells are significantly increased in the HRD group compared with the SH group. Serum IL-22 level is closely correlated with extent of hypertensive renal damage, indicating that serum IL-22 level may involve in the pathogenesis of hypertensive renal damage.