p27 在单侧输尿管结扎大鼠肾间质纤维化模型中的表达

目的：观察单侧输尿管梗阻（unilateral ureteral obstruction，UUO）大鼠模型中p27在肾小管中的表达，了解p27与肾间质纤维化程度的关系。方法：建立UUO大鼠模型，采用免疫组织化学方法检测UUO组及假手术组（Sham-operated rates，SOR）术后第7 d，14 d，21 d肾小管中p27的表达及其与肾间质纤维化程度的关系。结果：UUO组术后第7 d与SOR组比较，p27在肾小管上皮细胞中的表达无明显变化；术后第14 d UUO组p27的表达下降，与SOR组比较差异有显著性（P<0.05)；术后第21 d UUO组p27的表达明显下降，与SOR比较差异有显著性（P<0.01)。结论：p27在早期UUO大鼠模型中的表达无明显变化，随着间质纤维化程度的增加，p27的表达明显下降。

Expression of p27 in renal interstital fibrosis in ratsinduced by unilateral ureteral obstruction

Objective To explore the role of p27 in the renal tubule on the process of renal interstitial fibrosis caused by unilateral ureteral obstruction(UUO) in rats. Methods Rats were killed at the 7th, 14th, 21st days after UUO or sham-surgery. The protein expression of p27 in renal tubule was detected by immuohistochemistry at each time point. Results At the 7th day after the surgery, no noticeable abnormalities of p27 expression were found in renal tubular cells between UUO and the sham operated surgery. At the 14th and 21st days after UUO, the p27 expressions in renal tubular cells obviously decreased compared with those after the sham operated surgery. Conclusion The p27 expression has no noticeable changes in the early UUO rats, and then it was significantly reduced with the renal interstitial fibrosis progressing.