| 重组人肝细胞生长因子基因的克隆 |
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| 引用本文： | 罗建新,谭文斌,谭运年,成光杰.重组人肝细胞生长因子基因的克隆[J].中南大学学报(医学版),2000,25(5):425-427. |
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| 作者姓名： | 罗建新 谭文斌 谭运年 成光杰 |
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| 作者单位： | 1. 湖南医科大学,临床生化教研室
2. 湖南医科大学,分子生物学研究中心,长沙,410078 |
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| 基金项目： | 中国科学院资助项目,39700050, |
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| 摘 要： | 用RT PCR技术从人胎盘组织内成功地扩增全长肝细胞生长因子 (HGF)cDNA基因 (2 184bp) ,并将其克隆至pGEM T载体 ,经限制性核酸内切酶NdeⅠ ,Bg1Ⅱ ,HindⅢ ,BamHⅠ和XhoⅠ的酶谱分析和DNA测序分析证实。再将其亚克隆至逆病毒载体pLNL XHC ,可进一步用于基因表达和基因治疗的研究
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| 关 键 词： | 肝细胞生长因子 基因克隆 逆病毒载体 |
| 修稿时间： | 2000-07-31 |
Cloning of the recombinant human hepatocyte growth factor gene |
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| LUO Jian-xin,TAN Wen-bin,TAN Yun-nian,CHEN Guang-jie.Cloning of the recombinant human hepatocyte growth factor gene[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2000,25(5):425-427. |
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| Authors: | LUO Jian-xin TAN Wen-bin TAN Yun-nian CHEN Guang-jie |
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| Institution: | 1Department of Clinical Biochemistry; 2Research Center of Molecular Biology, Hunan Medical University(Changsha 410078) [ |
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| Abstract: | The full length of HGF cDNA gene(2?184?bp) was amplified successfully from human placental tissue using RT PCR technique, and then cloned into pGEM T vector, which was identified and confirmed by restriction endonuclease mapping using NdeⅠ, Bg1Ⅱ, HindⅢ, BamHⅠ and XhoⅠ, as well as DNA sequencing. The above clone of HGF cDNA gene was successfully subcloned into the retrovirus vector(pLNL XHC), which may be used for further studies of gene expression and gene therapy. |
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| Keywords: | hepatocyte growth factor gene clone retrovirus vector * |
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