特发性弱精子症患者精子中peroxiredoxin I与精浆活性氧的关系

目的：探讨peroxiredoxin I 在特发性弱精子症患者精子中的表达情况及其与精浆活性氧(reactive oxygen species，ROS)的关系。方法：选择2012年9月至12月在中南大学湘雅医院生殖医学中心就诊的男性不育患者。实验 组为26名特发性弱精子症患者，选取15名有生育史且精液分析各参数正常的男性为对照组，收集新鲜精液。应用鲁 米诺化学发光法检测精浆中ROS水平，蛋白质免疫印迹技术检测peroxiredoxin I在特发性弱精子症患者及对照组精子 中表达的差异。结果：1)实验组精浆ROS水平增高，与对照组相比差异有统计学意义(P<0.05)，两组患者精浆ROS 水平与前向运动精子活动率呈负相关(r=–0.777，P<0.01)。2)特发性弱精子症患者精子中peroxiredoxin I的表达显著低 于对照组(P<0.05)；两组患者精子peroxiredoxin I含量与精浆ROS水平也呈负相关(r=–0.474，P<0.01)。3)两组患者精子 peroxiredoxin I的含量与前向运动精子活动率呈正相关(r=0.779，P<0.01)。 结论： 精子中peroxiredoxin I表达下降可能是 引起特发性弱精子症发生的重要环节之一。高水平ROS可能是引起特发性弱精子症精子活力下降的主要原因之一。

Peroxiredoxin I in sperm and reactive oxygen species in seminal plasma in patients with idiopathic asthenozoospermia

Objective: To explore the relationship between peroxiredoxin I expression and seminal reactive oxygen species (ROS) in patients with idiopathic asthenozoospermia. Methods: Twenty-six infertile male patients were selected from the Reproductive Medical Center, Xiangya Hospital, from September to December in 2012. Fresh semen was collected from an experimental group (26 idiopathic asthenozoospermia patients) and a control group (15 men with fertility history and normal semen). Luminol chemiluminescence method was applied to detect the seminal ROS level. Western blot was used to detect the peroxiredoxin I expression. Results: 1)The seminal ROS level in the experimental group was significantly increased compared with that in the control group (P<0.05), and the seminal ROS level was negatively correlated with mobility of the sperm (r=–0.777, P<0.01). 2) Compared with the control group, the peroxiredoxin I expression was significantly downregulated in the experimental group (P<0.05). The content of sperm peroxiredoxin I in the 2 groups was negatively correlated with the seminal ROS level (r=–0.474, P<0.01). 3) The content of peroxiredoxin I had a positive correlation with human sperm motility(r=0.779, P<0.01). Conclusion: The decline of peroxiredoxin I expression may be one of the crucial factors that leads to idiopathic asthenozoospermia. High level of ROS may be one of the main reasons for sperm vitality decline in patients with idiopathic asthenozoospermia.