CD147siRNA对裸鼠恶性黑色素瘤生长的影响

目的:探讨CD147siRNA对恶性黑色素瘤成瘤及增殖活性和血管新生的影响.方法:建立pSUPER/CD147siRNA转染恶性黑色素瘤A375细胞皮下异种移植裸鼠模型并观察肿瘤生长情况;应用免疫组织化学方法检测裸鼠肿瘤组织内增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)和CD31的表达,依据PCNA的表达判断细胞的增殖活性,CD31的表达评价肿瘤微血管密度.结果:实验组成瘤率与对照组相比无统计学差异(P＞0.05),但实验组肿瘤体积显著缩小(P＜0.01).实验组PCNA的表达和血管密度明显低于对照组(均P＜0.01).结论:CD147 siRNA在裸鼠体内可显著抑制恶性黑色素瘤生长和血管新生,CD147分子可能是基因治疗恶性黑色素瘤一个新的靶分子.

Effect of pSUPER/CD147siRNA on the growth ofmalignant melanoma in nude mice

Objective To investigate the effect of pSUPER/CD147siRNA on the formation, proliferation, and angiogenesis of malignant melanoma in nude mice. Methods The nude mouse subcutaneous xenotransplantation models of malignant melanoma were constructed and observed using morphological analysis. The protein expression of PCNA and CD31 in tumors was detected using immunohistochemical technique. Tumoral proliferative activity in the nude mice was quantitatively evaluated by proliferation cell nuclear antigen (PCNA). Microvessel density( MVD) was counted based on the endothelial cells positively stained with anti-CD31 antibody. Results The subcutaneous tumors appeared in all the nude mice 5 days later after the transplantation, but the volume of malignant melanoma in the experiment group significantly decreased compared with the control group (P<0.01). PCNA expression and MVD were significantly lower in the experiment group than those in the control group ( P<0.01). Conclusion CD147 siRNA inhibits the growth and angiogenesis of malignant melanoma in vivo, suggesting that CD147 might be a new gene therapy target molecule for malignant melanoma.