首页 | 本学科首页   官方微博 | 高级检索  
检索        

裸鼠脾脏移植瘤模型在NGX6抗结肠癌转移作用研究中的应用
引用本文:肖志明,沈守荣,连平,王晓艳,刘芬.裸鼠脾脏移植瘤模型在NGX6抗结肠癌转移作用研究中的应用[J].中南大学学报(医学版),2007,32(5):753-757.
作者姓名:肖志明  沈守荣  连平  王晓艳  刘芬
作者单位:中南大学湘雅三医院消化内科,长沙 410013
基金项目:国家自然科学基金 , 湖南省自然科学基金 , 国家教育部博士专项科研基金
摘    要:目的:建立结肠癌肝转移模型,为抑瘤基因NGX6功能研究提供体内试验平台.方法:将低分化结肠癌细胞系HT-29组(HT-29)、空白质粒转染组pcDNA3.1( )/HT-29]、NGX6转染组(pcDNA3.1( )/NGX6/HT-29)]细胞分别接种于裸鼠脾脏下极包膜内,每天称量裸鼠体质量并观察裸鼠摄食、活动及精神情况,45 d后处死裸鼠,分别从大体水平以及镜下观察肝、脾、肺、肾、脑等器官肿瘤转移情况.结果:pcDNA3.1( )/NGX6/HT-29组裸鼠较其他两组裸鼠肝脏转移灶数目明显减少、脾脏上种植瘤形成明显减少.结论:抑瘤基因NGX6抑制结肠癌HT-29细胞的转移,裸鼠脾内种植转移模型可作为新基因体内功能研究的理想模型.

关 键 词:结肠癌  NGX6基因  裸鼠  动物模型  
文章编号:1672-7347(2007)05-0753-05
收稿时间:2006-9-29
修稿时间:2006-09-29

Intraspleenic tumor modle of nude mice in the anti-metastasis roles of NGX6 gene against colon cancer
XIAO Zhi-ming,SHEN Shou-rong,LIAN Ping,WANG Xiao-yan,LIU Fen.Intraspleenic tumor modle of nude mice in the anti-metastasis roles of NGX6 gene against colon cancer[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2007,32(5):753-757.
Authors:XIAO Zhi-ming  SHEN Shou-rong  LIAN Ping  WANG Xiao-yan  LIU Fen
Institution:Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha 410013, China
Abstract:Objective To establish a liver metastasis model of nude mice in colon cancer so as to determine the function of NGX6. Methods The cells of Group HT-29, pcDNA3.1( )/HT-29, and pcDNA3.1( )/NGX6/HT-29 were implanted into the spleen of nude mice, respectively. Everyday we measured the weight of the nude mice and observed their ingestion, movement and mental status. The nude mice were killed after 45 days, and the effect of NGX6 on the malignant behavior of HT-29 was assessed by this experiment. Results In contrast to the other two groups, the metastasis in the liver and xenograft tumor in the spleen of pcDNA3.1( )/NGX6/HT-29 group was significantly reduced (P<0.01). Conclusion The metastasis of HT-29 colon cancer cell line was significantly inhibited by NGX6 gene. This model of liver metastasis in the nude mice is a proper model to determine the anti-metastasis mechanism of NGX6 gene.
Keywords:colon cancer  NGX6 gene  nude mouse  animal model
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《中南大学学报(医学版)》浏览原始摘要信息
点击此处可从《中南大学学报(医学版)》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号