川芎嗪对大鼠急性脊髓损伤模型caspase-3和NF表达的影响

目的:观察川芎嗪对大鼠急性脊髓损伤模型损伤段半胱氨酸蛋白酶3(caspase-3)和神经丝蛋白(NF)表达的影响.方法:SD大鼠88只,随机分为空白对照组,生理盐水组和川芎嗪组.采用改良ALLEN氏打击法建立大鼠急性脊髓损伤模型.采用改良Rivlin斜板实验和BBB评分对大鼠脊髓功能进行行为学评分.在建模后1h,3h,6h,1d,3d,7d,14d和21d获取损伤段脊髓标本,HE染色观察其组织病理变化;免疫组织化学染色检测caspase-3和NF-L,NF-H,NF-M表达,并进行相关分析.结果:随着时间推移,术后斜板临界度数和BBB评分均逐渐升高,且术后7,14和21d,川芎嗪组斜板临界度数和BBB评分均较对照组高(P＜0.05).术后3,7和14d,川芎嗪组caspase-3表达值较生理盐水组低(p＜0.05),NF表达值较生理盐水组高(P＜0.05).改良Rivlin斜板临界角度与NF的表达正相关:BBB评分值与NF的表达正相关,与caspase-3的表达负相关;Caspase-3的表达与NF的表达负相关.结论:川芎嗪对大鼠急性脊髓损伤模型损伤段脊髓有保护作用,可能与川芎嗪增加NF表达,抑制caspase-3表达有关.

Tetramethylpyrazine accelerated spinal cord repair through regulation of caspase-3 and neurofilament protein expression:an acute spinal cord injury model in rats

Objective To investigate the effect of tetramethylpyrazine on spinal cord repair through hypothesized pathways including regulation of caspase-3, neurofilament protein NF-L, NF-H, and NF-M expression.Methods Allenos weight drop method was used to establish acute spinal cord injury (SCI) rat model at T10 section and was conducted in 80 adult Sprague Dawley rats that were subsequently divided into pseudo-therapy group (sodium chloride group, n=40) and tetramethylpyrazine group (treatment group, n=40). Eight normal rats were included into the pseudo-surgery normal group (n=1 for each time point). The function of spinal cord was evaluated with animal behavioral scores by measuring modified Rivilin loxotic plate degree and counting BBB score at 1, 3, 7, 14, and 21 d postoperatively. The injured spinal cord tissue samples were harvested at 1 h, 3 h, 6 h, 1 d, 3 d, 7 d, 14 d, and 21 d postoperatively (n=5 for each time point) for the preparation of continuous histological sections that were analyzed the expression of caspase-3,NF-L,NF-H, and NF-M by immunohistochemistry method.Results At postoperative Day 7, 14, and 21, animal behavioral scores revealed higher modified Rivlin loxotic plate degree and BBB scores in the treatment group when compared with those of the control group (P<0.05). The cells in the injured spinal cord tissue expressed significantly less caspase-3 and significantly more NF (NF-L,NF-H and NF-M) in the treatment group than those in the control group at Day 3, 7, and 14 (P<0.05). There was positive correlation between modified Rivlin loxotic plate degree and NF expression, BBB score and NF expression; and negative correlations between BBB score and caspase-3 expression, caspase-3 expression and NF expression.Conclusion Tetramethylpyrazine improves spinal cord healing through regulation of caspase-3 and neurofilament protein expression.