救心复脉注射液对豚鼠心室肌细胞L型钙电流的影响

目的：探讨救心复脉注射液(枳实提取液)有效成分辛弗林(311)、N-甲基酪胺(417)对豚鼠心室肌细胞膜L型钙通道电流（ICa-L）的影响。方法：用酶解法分离单个心室肌细胞，采用全细胞膜片钳技术，观察辛弗林、N-甲基酪胺对豚鼠心室肌细胞膜ICa-L的影响。结果：用辛弗林（10，25，50，100 mmol/L)时能增大 ICa-L ，增加率分别为 8.27%，27.29%，41.01% 和 48.74% (P<0.05)。 用N-甲基酪胺（10，25，50，100 mmol/L） 时能增大 ICa-L ，增加率分别为 10.05%, 30.12%，43.05% 和51.90%(P<0.05)。辛弗林和N-甲基酪胺只改变电流幅度，不改变I-V曲线形状。结论：辛弗林和N-甲基酪胺有浓度依赖性的增大豚鼠心室肌细胞ICa-L，促进钙通道开放的作用。

Effect of Jiuxinfumai injection on L-type calcium currents in single guinea pig ventricular myocytes

Objective To determine the effect of 311 and 417, both active ingredients isolated from Jiuxinfumai injection (Citrus Aurantium) on L-type calcium currents (I Ca-L)in ventricular myocytes of guinea pigs.Methods Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of 311and 417. Results 311(10,25,50,100 mmol/L)increased the I Ca-L by 8.27%, 27.29%, 41.01%,and 48.74% (P<0.05), respectively. 417(10,25,50,100 mmol/L) increased the I Ca-L by 10.05%, 30.12%,43.05%,and 51.90% (P<0.05), respectively. Both 311 and 417 changed the I Ca-L significantly in a concentration-dependent manner. They did not change the shape of I-V cruves.Conclusion 311 and 417 can increase I Ca-L in ventricular myocytes of guinea pigs in a dose-response manner.