CD15nRNA,CD44v6mRNA及nm23H1mRNA在乳腺癌组织中的表达及 …

目的 探讨ＣＤ１５ｍＲＮＡ及其蛋白、ＣＤ４４ｖ６ｍＲＮＡ和ｎｍ２３Ｈ１ ｍＲＮＡ在乳腺癌组织中的表达及与乳腺癌患者预后的关系。方法 应用催化信号放大（ＣＳＡ）原 杂交和免疫组织化学方法,对９４例乳腺癌患者的癌组织进行了ＣＤ１５ ｍＲＮＡ及其蛋白、ＣＤ４４ｖ６ ｍＲＮＡ和ｎｍ２３Ｈ１ ｍＲＮＡ低表达均与乳腺癌患者的组织学分级、临床分期、淋巴结转移、复发和预后密切相关。ＣＤ１５ ｍＲＮＡ和ＣＤ４４ｖ６

The expression of CD15 mRNA CD44v6 mRNA and nm23H1 mRNA in breast cancer and their clinical significance

OBJECTIVE: To study the relationship of the expression of CD15 mRNA and its protein, CD44v6 mRNA and nm23H1 mRNA with the clinical pathology parameter and prognosis of breast cancer, and to investigate the correlation of the expression of CD15 mRNA with CD44v6 mRNA and nm23H1 mRNA. METHODS: Catalyzed signal amplification (CSA) method of in situ hybridization and immunohistochemistry were used to detect the expression of CD15 mRNA and its protein, CD44v6 mRNA and nm23H1 mRNA in 94 cases of breast cancer. RESULTS: The overexpression of CD15 mRNA and its protein and CD44v6 mRNA and the low expression nm23H1 mRNA were correlated with the grading, clinical stage, lymph node metastasis, recurrence and prognosis of breast cancer. Patients who had overexpression of CD15 mRNA and CD44v6 mRNA and low expression of nm23H1 mRNA had a higher lymph node metastatic rate and a lower survival rate. CONCLUSION: The expression of CD15 mRNA has a synergistic action in positive and negative regulation with that of CD44v6 mRNA and nm23H1 mRNA. Combining detection of the expression of these three mRNA is a reliable index to evaluate the metastasis, recurrence and prognosis of breast cancer.