日本血吸虫单克隆抗独特抗体NP30对山羊的保护性免疫作用

目的 观察日本血吸虫克隆抗独特型抗体ＮＰ３０主动免疫对山羊的保护性免疫作用。方法 将２２只山羊随机分为两组：（１）实验组：１２只，每只山羊 ＮＰ３０的免疫剂量为１０００μｇ／次，后腿肌肉注射，连续免疫３次，最后１一８周进行尾蚴攻击。（２对照组：１０只，肌肉注射ＳＰ２／０４腹水。尾蚴攻击感染后第１２ 睛死。结果 ＮＰ３０主动免疫山羊可放 减虫率，肝组织减弱率为３５．８３％，粪减卵率为２５％，并可明显

Protective immunity induced by anti idiotypic monoclonal antibody NP30 of Schistosoma japonicum in goats

OBJECTIVE: To observe the protective immunity in goats induced by active immunization with anti-idiotypic monoclonal antibody NP30 of S. japonicum. METHODS: Goats were randomly divided into two groups. Experimental group had 12 goats. The NP30 was intramuscularly injected with a dosage of 1 000 microgram into the hind leg for three dosages continuously. The goats were challenged with cercariae eight weeks after the third immunization. Ten goats in the control group were intramuscularly injected with SP2/0 ascites and challenged with cercariae at the same time as the experimental group. All goats were killed at the twelfth week post-challenge. RESULTS: Active immunization with NP30 could produce a protection rate of 42.78% in goats. The number of eggs in liver and feces in the goats immunized with NP30 was decreased by 35.83% and 25%, respectively. The size and number of egg granulomas in the liver were lessened obviously and the weight of the goats increased significantly. CONCLUSION: Active immunization with NP30 could induce a good protection in goats against the infection with Schsitosoma cercariae.