女性生殖道中新型致病支原体的分离及其临床意义

目的：了解我国妇女生殖道感染性疾病患者中生殖道是否存在新型致病 支原体（穿通支原体、发酵支原体和梨支原体）的感染，为临床诊治提供依据。方法：采集女性生殖道感染患者和正常人（各172例）的宫颈分泌物，接种在改良的SP－4培养基，进行分离培养。阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA序列测定等方法进行鉴定。结果：从172例患者宫颈分泌物中分离到1株穿通支原体（0．58％），2株发酵支原体（1．16％）。从172名正常女性的宫颈分泌物中没有分离到相应的菌株。结论：从病原学首次证实，南京地区女性生殖道感染性疾病患者中存在穿通支原体、发酵支原体的感染。

New type pathogenic mycoplasmas isolated from female patients with genital infection in Nanjing, China

Objective To study if infection of new type pathogenic mycoplasmas, Mycoplasma penetrans (Mpe), Mycoplasma fermentans (Mf), and Mycoplasma pirum (Mpi), exists in the genital tract of gynecologic patients with genital infection in China so as to provide information for clinical diagnosis and therapy. Methods Cervical secretions from 172 patients with infectious diaseses of gentital tract and 172 healthy controls were collected and inoculated in improved SP 4 culture medium. Biochemical reaction, coagglutination test, metabolism inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms. Results One strain of Mycoplasma penetrans (0.58%) and two strains of Mycoplasma fermentans (1.16%) were isolated from the cervical secretions of patients, but none was found in healthy controls. Conclusion Mpe infection and Mf infection exist in female patients with genital infection in Nanjing.