天疱疮抗体对桥粒影响的动态观察

目的 观察天疱疮抗体对表皮角朊细胞粘合性连接（桥粒及其成分ｄｇ２／３）的影响，探讨棘层松解过程中桥粒的动态变化情况。方法采用纯化的天疱疮抗体，建立天疱疮的器官培养模型和棘层松解模型，观察加入天疱疮抗体后不同时相表皮在光学显微镜下和电子显微镜下的结构情况，并用抗ｄｇ２／３单克隆抗体进行免疫组织化学染色。结果天疱疮抗体在２４小时内仅引起细胞间隙增宽；在４８小时时可引起部分桥粒的破坏；７２小时时可致胞膜变平滑，桥粒消失。免疫组织化学检测结果表明有桥粒成分的内部化。结论天疱疮抗体对桥粒及其成分的影响是在较晚期开始的，但桥粒的破坏是棘层松解的关键一环。

A time-course observation of influence of pemphigus autoantibody upon desomosome.

Objective To observe the time-course changes of desmosome and its dg2/3 component in the pro- cess of acantholysis. Method After setting up apemphigus organ model and an acantholysis model by purified pemphigus autoantibody , the desmosome structures at light and electromicroscopic levels and desmoglycoprotein dg2 /3 immunohistochemical stains were observed at different culture times. Resul ts ln pemphigus organ model and acantholyais model , pemphigus autoantibody only induced the increase of the intercellular space at 24 hour. Even at 48 hour , only a few desmosomes were damaged. But by 7 2 hour , the structures of desmosomes almost completely disappeared , and the cell membrane flattened. Immunohistochemical stains showed that there was an internalization of desmoglycoprotein dg2 /3 at 4 8 and 72 hour during the process of acantholysis. Conclusion Our results suggested that the influence of pemphigus autoantibody on desmosomes appears at a relative later stage , but the damage of desmosomes is a pivotal step to acantholysis.